摘要
目的 探讨蛛网膜下腔出血（SAH）后早期脑损伤和自噬激活的相关性.方法 比较SAH后大鼠脑含水量变化以及双重免疫荧光染色结果.结果 脑干湿重法显示SAH后大鼠6h大脑半球含水量为78.25％,3d时为79.75％,明显增高（P＜0.05）；小脑半球在各时间点含水量差异无统计学意义（P＞0.05）.胶质纤维酸性蛋白和Beclin-1染色显示神经元细胞Beclin-1染色呈阳性,自噬激活发生于神经元细胞；Beclin-1和微管相关蛋白2染色显示神经元细胞呈阳性,神经元细胞自噬被明显激活,与含水量变化表现为相同的时程变化.结论 大鼠SAH后出现了大脑半球早期脑损伤表现和自噬激活征象,自噬可能在SAH后早期脑损伤过程中起关键作用.
Objective To investigate the expression of glial fibrillary acidic protein （GFAP）,Beclin-1 and microtubule-associated protein 2 （MAP2） after subarachnoid hemorrhage （SAH） in rats,and explore the relationship between early brain injury and autophagy in the process of SAH.Methods A total of 40 adult male SD rats were randomly divided into sham group and SAH 6 h,24 h and 3 day groups.Rats were decapitated under anesthesia after SAH induction.The brains were immediately removed and separated into hemispheres and cerebellums.The water content of each brain sample was weighed.A total of 10 adult male SD rats were decapitated under anesthesia after 3 days of SAH induction.The expression of GFAP,Beclin-1 and MAP2 was observed by double-labeled immunofluorescent staining.Results Following SAH induction,water content of the hemispheres was globally elevated,which was 78.25% at 6th h after induction,and the increase was most significant at the 3rd day （79.75%,P 〈 0.05）.There was no significant difference in water content of the cerebellums among the different time points.Double-labeled immunofluorescent staining of GFAP/Beclin-1 showed that autophagy was expressed predominantly in neurons.And double-labeled immunofluorescent staining of Beclin-1/MAP2 showed that autophagy was obviously activated in neurons,which coordinated with the edema of brain.Conclusion The brain of rats showed the performance of early brain injury after SAH,and the neurons of hemisphere had the sign of autophagy activation in early phase.Autophagy may play a key role in the process of early brain injury after SAH.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第11期2336-2338,共3页
Chinese Journal of Experimental Surgery
基金
浙江省医药卫生科学研究基金资助项目（2010KYB130、2012KYA191）
