摘要
目的 制备一种新型的人工骨支架材料,并探讨其生物相容性.方法 采用溶液共混法制备了壳聚糖-海藻酸钠-羟基磷灰石骨（CS/ALG/n-HAP）复合生物支架材料.将兔股关节软骨细胞接种于复合支架在96孔板中培养3d后,噻唑蓝（MTT）比色法测定软骨细胞的增殖.结果 软骨细胞活性与CS/ALG/n-HAP复合材料中n-HAP含量有关,当CS/30％ ALG复合材料中含3％n-HAP最适合软骨细胞生长,细胞生长相对百分率为63.45％;5％ n-HAP的CS/30％ ALG表面颗粒增多,细胞相对增殖率降至38.39％.结论 CS/ALG/n-HAP复合支架材料无毒,生物相容性好,有良好的研究应用前景.
Objective To prepare a kind of novel artificial scaffold and study its biocopatibility.Methods The chitosan/alginate/hydroxyapatite （CS/ALG/n-HAP） composites scaffold was prepared by using solution blending.Rabbit hip chondrocytes were seeded on the scaffolds for compound culture.Cellscaffold compounds were cultured in 96-well plates for 3 days,and then chondrocyte proliferation was determinded by methyl thiazol tetrazolium （MTT） assay.Results The proliferation reactivity of the chondrocytes was related to the content of n-HAP in CS/ALG/n-HAP composites scaffold.CS/30% ALG/n-HAP composites scaffold containing 3% n-HAP was ideal for the growth of the chondrocytes with the proliferation rate of the chondrocytes being 63.45%.However,CS/30% ALG/5% n-HAP composites scaffold had more particles on the surface and the proliferation rate of the chondrocytes was lowered to 38.39%.Conclusion CS/ALG/n-HAP artificial scaffold is cell non-toxic and has good biocompatibility.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第11期2374-2376,共3页
Chinese Journal of Experimental Surgery
基金
福建卫生厅中医药重点课题（wzzg0902）
