摘要
目的 探讨RNASET2在胰腺癌中的表达及意义.方法 采用逆转录-聚合酶链反应（RT-PCR）法检测胰腺癌（32例）、癌旁正常组织（24例）、人胰腺癌细胞株（3株）和正常导管上皮细胞株中RNASET2的表达,采用Western blot法和免疫组织化学（IHC）方法分别检测胰腺癌冰冻组织（8例）和石蜡包埋组织（10例）中RNASET2的表达.结果 RNASET2在胰腺癌表达明显下降（6.516 ±3.033,P＜0.05）,在人胰腺癌细胞株BxPC-3、PANC-1和SW1990中表达明显下降（2.814±0.325、2.342±0.454和5.473±0.682,P＜0.01）；胰腺癌组织和细胞株中RNASET2蛋白质表达相对下降；胰腺癌神经侵犯者癌组织中RNASET2表达更低（5.021±3.102,P＜0.05）.结论 RNASET2在胰腺癌组织和细胞株中表达下降,可能与胰腺癌发生和神经侵犯相关.
Objective To investigate the expression of RNASET2 in pancreatic cancer and its sig- nificance. Methods The expression levels of RNASET2 mRNA and in pancreatic cancer （32 cases）, ad- jacent benign tissue （24 cases）, 3 human pancreatic cancer cell lines and benign ductal epithelial cell line were quantitated by using reverse transcriptase-polymerase chain reaction （RT-PCR）. The protein expres- sion of RNASET2 was evaluated by Western blotting in 8 fresh frozen tissues and immunohistochemistry （IHC） in 10 paraffin-embedded specimens. Results RNASET2 was down-regulated significantly in pan- creatic cancer （6. 516 ±3. 033,P 〈0. 05） and cell lines BxPC-3, PANC-1 and SW1990 （2. 814±0. 325, 2. 342 ± 0. 454 and 5. 473± 0. 682,P 〈 0. 01 ） respectively. The protein expression of RNASET2 was obvi- ously down-regulated in pancreatic cancer tissues and tumor cell lines. The group with perineural invasion showed lower expression of RNASET2 （5. 021±3. 102,P 〈 0. 05 ）. Conclusion The expression of RNA- SET2 was down-regulated in pancreatic ductal adenocarcinoma and tumor cell lines, and this gene might be correlated to tumorigenesis and ~erineural invasion of pancreatic cancer.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第11期2417-2419,F0004,共4页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（81172274）
上海市科学技术委员会资助项目（11JC1401600）
