摘要
目的：建立目舒丸的定性定量方法.方法：采用TLC对目舒丸中白芍、延胡索进行定性鉴别;采用HPLC对目舒丸中芍药苷进行含量测定,色谱条件为ZORBAX SB-C18色谱柱（4.6 mm×150 mm,5μm）,流动相乙腈-0.1％磷酸水（15：85）,流速1.0 mL·min-1,检测波长230 nm,进样量10μL,柱温30℃.结果：白芍、延胡索乙素的TLC鉴别简便可靠.芍药苷在37.8～756 ng呈良好线性关系,平均回收率98.2％,RSD 1.4％.结论：建立的方法准确、可靠、专属性强,可用于该制剂的质量控制.
Objective： To develop the qualitative and quantitative methods for quality control of Mushu pills. Method： TLC were used as qualitative identifications of Paeoniae Radix Alba and Corydalis Rhizoma in Mushu pills; And to develop a HPLC method for determination of paeoniflorin in Mushu pills, separation was performed on ZORBAX SB-Cls column （4.6 mm ×150 mm, 5μm） with acetonitrile-0. 1% phosphoric acid （15： 85） as mobile phase, flow rate of 1.0 mL min-1, detection wavelength was at 230 nm, injection volume 10 μL, column temperature was 30 %. Result： TLC characteristic identification was distinct and highly specific. Paeoniflorin showed a good liner relationship within the range of 37.80-756.00 ng, the average recovery was 98.2% with RSD 1.4%. Conclusion： This established determination method was reliable, accurate and specific, it could be used for quality control of Mushu pills.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第22期67-70,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中国中医科学院眼科医院院内课题(201109)
关键词
目舒丸
白芍
延胡索
芍药苷
薄层色谱鉴别
高效液相色谱
Mushu pills
Paeoniae Radix Alba
Corydalis Rhizoma
paeoniflorin
TLC identification
HPLC
