摘要
目的探讨消化道肿瘤多药耐药基因MDR1基因和P-糖蛋白(P-gp)的表达与肿瘤化疗药物耐药之间的关系。方法选择72例消化道肿瘤患者的手术标本,采用荧光定量PCR检测MDR1 mRNA表达量,ELISA法检测P-gp表达水平,并与ATP-TCA化疗药物敏感性试验结果作对照。结果 72份消化道肿瘤组织中,MDR1 mRNA阳性表达率为75%,P-gp阳性表达率为67%。72份消化道肿瘤组织中,对10种化疗药物均不敏感的有12份,对1种化疗药物敏感的有15份,对2种化疗药物敏感的9份,对3种化疗药物敏感的有9份,对5种化疗药物敏感的有15份,对8种化疗药物敏感的有9份,对所有化疗药物均敏感的有3份。消化道肿瘤MDR1mRNA、P-gp的阳性表达率与其对化疗药物敏感程度呈负相关(r s值分别为-0.672、-0.715,均P<0.05)。结论 MDR1基因、P-gp表达与肿瘤耐药性相关,MDR1基因、P-gp阳性表达提示患者可能对化疗药物存在获得性耐药。
Objective To investigate the correlations between the expression of muhidrug resistance gene 1 （ MDR1 ） gene and P - glyeoprotein （ P - gp） and chemotherapy drug resistance of gastrointestinal （GI） tumor. Methods The expression of MDR1 mRNA and P - gp in 72 patients with GI cancer was measured by RT - PCR and ELISA. The chemotherapy drug sensitivity test was also conducted for eorrelaion analysis. Results The positive rates of MDR1 and P -gp in GI tumor tissue were 75% and 67%, respectively. There were 12 eases insensitive to 10 chemotherapy drugs; while singledrug, 2 -drug, 3 -drug, 5 -drug, 8 -drug and all- drug sensitive were revealed in 15, 9, 9, 15, 9 and 3 eases, respectively. The positive expression of MDR1 and P - gp was significantly negatively correlated with the sensitivity to chemotherapy in GI cancer （ rs = - 0. 672 and - 0. 715, P 〈 0. 05 ）. Conclusion The expression of MDR1 and P - gp is correlated to the drug resistance of GI tumor, which indicates functional linkage of drug resistanee.
出处
《广东医学》
CAS
CSCD
北大核心
2013年第20期3120-3123,共4页
Guangdong Medical Journal
基金
广东省自然科学基金面上项目(编号:10151031701000009)
