摘要
目的掌握云浮国境口岸蚊类携带重要蚊媒病毒的现状，探讨提高口岸蚊媒病毒检测有效性的方法，为预防和控制口岸蚊媒传染病的发生提供科学依据。方法于2011年5月～11月在云浮口岸捕捉活蚊，对蚊标本研磨液提取核酸后应用多重实时荧光RT-PCR技术快速筛查蚊类携带的登革热病毒、日本脑炎（乙脑）病毒、基孔肯雅病毒、西尼罗热病毒和黄热病毒。对实时荧光RT—PCR检测核酸阳性的标本进行PCR扩增和序列分析。结果在云浮共采集39254只蚊虫，鉴定后分为460组。经宾时荧光RT—PCR检测，14组蚊虫乙脑病毒核酸阳性，其余病毒（登革热病毒、乙脑病毒、基孔肯雅病毒、西尼罗热病毒和黄热病毒）均为阴性。PCR扩增和序列分析显示云浮乙脑病毒属于基因I型。所捕获的三带喙库蚊的最小感染率为0．937‰比中华按蚊的0．385％。稍高。结论本研究首次在云浮口岸进行大规模系统的蚊媒病毒调查，云浮口岸的蚊虫很可能没有携带登革热病毒、基孔肯雅病毒、西尼罗热病毒和黄热病毒，但有少数蚊虫携带乙脑病毒。此次调查检出的基因I型乙脑病毒是广东省内的首次报道，为今后蚊媒病毒的媒介监测检测工作提供了有效方法。虽然蚊虫最小感染率的差别没有统计学意义，但是蚊媒病毒病的暴发和流行风险仍然存在。
Mosquito samples were collected at Yunfu Port in China and detected for representative mosquito-borne arbo- viruses during May to November of 2011. Representative mosquito-borne arboviruses, including dengue, Japanese encephalitis （JE）, ehikungunya, West Nile, and yellow fever viruses, were detected by multiple real-time fluorescence PCR assay. A fur- ther study of PCR amplification and sequence analysis to the positive samples were performed. A total of 39 254 individual mos- quitoes were collected from 460 mosquito pools at Yunfu Port. All the virus including dengue, chikungunya, West Nile, and yellow fever viruses showed negative results except for 14 positive results with JE virus （JEV） nucleic acid detected by real- time PCR assay. The genotype of JEV was confirmed as genotype I by PCR amplification and sequence analysis of E gene. The genotype I JEV carried by mosquito was found for the first time in Guangdong. The minimum infection rate （MIR） of JEV in Culeac tritaeniorhynchus was 0. 9370/oo0, which was slightly higher than that in Anopheles sinensis at 0. 385%o at Yunfu Port. Although the difference in MIR was not statistically significant, the outhreak and epidemic risk of mosquito home arbovi ral disease still exists. The primary investigation of mosquito borne arbovirus at Yunfu Port would be useful to offer an effec- tive method for future mosquito borne arbovirus surveillance and detection.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2013年第11期1044-1049,共6页
Chinese Journal of Zoonoses
基金
Funded by the General Administration of Quality Supervision,Inspection and Quarantine of China(Nos.2004IK126-9&2013IK237)~~
关键词
口岸
病毒监测检测方法
蚊媒病毒
流行性乙型脑炎病毒
port
virus surveillance and detection methods
mosquito borne arbovirus
Japanese encephalitis virus
