期刊文献+

酸角叶中总黄酮的提取及清除羟自由基的作用研究 被引量:2

Study on Extraction of Flavonoids from Tamarind Leaves and Scavenging Effect on Hydroxyl Radicals
下载PDF
导出
摘要 [目的]优选酸角叶中总黄酮的提取条件,并对其清除羟自由基的作用进行研究。[方法]采用乙醇回流法提取酸角叶中的总黄酮,通过单因素确定总黄酮提取的较佳试验条件,并对总黄酮的清除羟基自由基(·OH)的作用进行对比试验。[结果]确定酸角叶中总黄酮的最佳提取条件为:乙醇浓度为70%,料液比为1∶30(W/V,g/ml,下同),回流温度为85℃,提取时间为2.5 h;在此条件下,酸角叶中总黄酮的提取率为1.83%。羟基自由基(·OH)的清除作用对比试验表明,抗氧化活性大小依次为:提取液中总黄酮>芦丁>BHT。[结论]该方法优选了酸角叶中总黄酮的最佳测定条件,并发现其对羟自由基有较强的清除作用。 [ Objective ] The aim was to study extraction of flavonoids and scavenging effect on hydroxyl radicals [ Method] Flavonoids were ex- tracted from tamarind leaves by using ethanol reflux method from tamarind leaves, and the preferred extraction conditions were determined by sin- gle factor experiment. [ Result] The study showed that the best extraction conditions was ethanol concentration of 70%, solid-liquid ratio of 1:30 (mg/ml), reflux temperature of 85 ~C and extraction time of 2.5 h. The extraction ratio of flavonoids was 1.83% by using spectrophotometry. Antioxidant effects of flavonoids, rutin and BHT were measured to remove hydroxyl radicals by comparative experiments. Antioxidant activity was ordered as flavonoids 〉 rutin 〉 BHT. [ Conclusion] Antioxidant experiments showed that flavonoids from tamarind leave had a strong scavengirtg effect on hydroxyl radicals.
作者 李会端 王仪
出处 《安徽农业科学》 CAS 2013年第23期9612-9614,9674,共4页 Journal of Anhui Agricultural Sciences
基金 云南省应用基础研究项目(2012FD050) 楚雄师范学院科学研究基金项目(11YJGG01)
关键词 酸角叶 总黄酮 乙醇回流法 清除效果 羟自由基 Tamarind leaves Flavonoids Ethanol reflux Scavenging effect Hydroxyl radicals
  • 相关文献

参考文献7

二级参考文献69

共引文献153

同被引文献31

引证文献2

二级引证文献20

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部