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Cloning and Expression Analysis of THWRKY12 Gene from Tamarix hispida

Cloning and Expression Analysis of THWRKY12 Gene from Tamarix hispida
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摘要 [ Objective] This study aimed to clone and analyze the expression of THWRKY12 gene in Tamarix hispida. [Method] T. hispida seedlings were treated with 400 mmol/L NaC1 solutlon, 20% PEG and 100 μmol/L ABA, respectively. The expression of THIVRKY12 gene in different tissues was investigated by RT-PCR technology. [Result] Under treatments of NaC1 and PEG, the expression of THWRKY12 gene in different tissues of T. h/sp/da seedlings showed an overall upregulated trend, suggesting that THWRKYI2 is related with the saline-alkali resistance and drought resistance of T. hispida. Under ABA treatment, THWRKY12 gene had approximately the same expression pattern with the former two, indicating that THWRKY12 gene may involve in the regulation and control of the saline-al- kali resistance and drought resistance of T. hispida through the signal pathway regulated by ABA. [ Conclusion] This study laid foundation for investigating the functions of WRKY gene in the stress resistance of T. hispida. [ Objective] This study aimed to clone and analyze the expression of THWRKY12 gene in Tamarix hispida. [Method] T. hispida seedlings were treated with 400 mmol/L NaC1 solutlon, 20% PEG and 100 μmol/L ABA, respectively. The expression of THIVRKY12 gene in different tissues was investigated by RT-PCR technology. [Result] Under treatments of NaC1 and PEG, the expression of THWRKY12 gene in different tissues of T. h/sp/da seedlings showed an overall upregulated trend, suggesting that THWRKYI2 is related with the saline-alkali resistance and drought resistance of T. hispida. Under ABA treatment, THWRKY12 gene had approximately the same expression pattern with the former two, indicating that THWRKY12 gene may involve in the regulation and control of the saline-al- kali resistance and drought resistance of T. hispida through the signal pathway regulated by ABA. [ Conclusion] This study laid foundation for investigating the functions of WRKY gene in the stress resistance of T. hispida.
作者 Jingxue WANG Yuekun TANG Qi LIU Chunqiang LIANG Yucheng WANG
机构地区 State Key Laboratory of Forest Genetics and Tree Breeding
出处 《Agricultural Biotechnology》 CAS 2013年第5期55-57,共3页 农业生物技术（英文版）
基金 Supported by College Students Innovative Experimental Project of Northeast Forestry University(No.201210225004)
关键词 Tamarix hispida WRKY transcription factor RT-PCR Saline-alkali stress Drought stress Tamarix hispida WRKY transcription factor RT-PCR Saline-alkali stress Drought stress
分类号 S793.5 [农业科学—林木遗传育种]
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Agricultural Biotechnology
2013年 第5期

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