摘要
目的 :克隆人 CCR5 5′侧翼调控序列及其基序分析。方法 :设计单引物 ,利用单向多循环 PCR扩增及聚丙烯酰胺凝胶电泳纯化人 CCR5 5′侧翼调控序列基因组 DNA,PCR产物作为序列分析模板。 结果 :得到长为 1.5 kb的人 CCR5 5′侧翼调控序列基因组 DNA序列 ,并对该区域的基序进行了分析。结论 :该方法适合于基因组 DNA,特别是逆向扩增一些读框区 5′侧翼调控序列。
Objective: To clone and analyze the human CCR5 5′ flank regulation sequence and its motifs. Methods: We designed single primer and amplified the genomic DNA sequence of human CCR5 5′ flank regulation domain with single primer PCR and multiple cycles method. The PCR product as a sequence template was purified by polyacrilamid electrophoresis. Results: A 1.5 kb genomic DNA fragment which including CCR5 5′ flank regulation sequence was obtained, and the motifs were analyzed. Conclusion: This method is suitable for genomic DNA of ORF 5′ upsteam regulation flank sequence amplification. It also can provide materials for gene regulation research. [
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2000年第11期1059-1061,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金资助项目 ( 3 9770 689)
关键词
调控序列
CCR5
基序分析
克隆
regulation sequence
human monocyte chemotactic receptor 5
motif analysis
