摘要
通过高效液相色谱法（HPLC）建立一种有效的测定人体血液中哌拉西林浓度的方法，为临床上合理使用哌拉西林提供理论依据。采用WatersC1s柱（250mmx4．6mm，5txm），流动相为乙腈-0．11％四丁基氢氧化铵溶液，卜安息香肟为内标，紫外检测器进行检测。实验结果显示哌拉西林的线性范围为0．5-100μg·mL-1，低、中、高3个浓度日内、日间精密度均小于5％，表明该方法灵敏度高，重现性好，可用于临床中哌拉西林血药浓度的测定以及临床药动学的研究。
The High Performance Liquid Chromatography （HPLC） method was established for the ef- fective determination of piperacillin in human plasma, thus we will provide theory basis for using pip- eracillin in the clinical. A Waters C18 column （250 mmx4.6 mm, 5 ~m） was used with the mobile phase of acetonitrile and 0. 11% tetrabutylammonium hydroxide solution. 1-Benzoin oxime was used as internal standard. Ultraviolet detector was employed. The results showed that the calibration curve of piperacillin was linear in the rang of 0. 5 - 100 Ixg ＂ mL-1. RSDs of the inter-day and intra-day precisions of low, medium and high concentrations were all less than 5%. So the method is sensitive, specific, repeatable and accurate for the determination of piperacillin in human plasma, and is availa- ble for the research about Clinical Pharmacokinetics.
出处
《实验室科学》
2013年第5期39-41,45,共4页
Laboratory Science
