摘要
以不同来源、不同类型的46个烟草品种为试验材料,进行抗烟草普通花叶病(TMV)特性的田间鉴定和分子鉴定,为筛选抗病烟草种质奠定基础。结果表明,22个品种的烟草抗TMV特性田间鉴定结果与前人鉴定结果基本相同,从24个前人未鉴定的烟草品种中初步筛选出14个中抗品种,8个中感品种和2个感病品种;通过抗TMV基因(CN)的特异引物进行扩增,35个烟草品种的基因组DNA具有特异性片段;通过分子鉴定和田间鉴定的结果比较,在具有CN或其同源基因35个烟草品种中62.9%田间鉴定表现为抗或中抗,83.3%的具有CN或其同源基因的选育烤烟品种田间鉴定表现为抗或中抗;结合两种方法,初步确定了3个中感品种和11个中抗品种。
In this study,46 different types and sources of tobacco germplasms were selected as experiment ma- terials to screen disease resistant tobacco varieties through field investigation and molecular identification of resist- ance ability to TMV. The results of field investigation showed that 14 moderate resistant tobacco germplasms,8 mod- erate susceptible germplasms,and 2 susceptible ones were sereened out from 24 unidentified tobacco geramplasms, while 22 tobacco geramplasms were resistant to TMV which basically the same as previous results. Furthermore,spe- cific fragments were amplified in 35 tobacco genomic DNA by PCR method of specific primers of TMV-resistant gene （CN）. The results showed that 62.9% of 35 tobacco germplasms and 83.3% of bred varieties which had CN gene or homologous gene were resistant or moderate resistant to TMV by field investigation and molecular identifica- tion. Finally,3 moderate susceptible and 11 moderate resistant tobacco geramplasms were identified by combining both the above two methods.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2013年第6期1173-1178,共6页
Journal of Plant Genetic Resources
基金
福建省自然科学基金项目(2010J01070)
福建省烟草专卖局资助项目(闽烟科2008(13)号)
关键词
烟草
TMV
抗花叶病
鉴定
Tobacco
tobacco mosaic virus （TMV）
resistance to TMV
identification
