摘要
目的探讨膜联蛋白A2在瘢痕疙瘩、增生性瘢痕和正常皮肤组织中的表达,初步探讨其在瘢痕疙瘩中的作用和意义。方法收集瘢痕疙瘩、增生性瘢痕各25例标本,正常皮肤组织10例,应用免疫组化、Western blot及荧光定量PCR法检测膜联蛋白A2在瘢痕疙瘩、增生性瘢痕和正常皮肤组织中的表达,通过图像分析系统定性并定量分析膜联蛋白A2在不同组织中表达的差异性。结果免疫组化、Western blot与荧光定量PCR结果均显示:与正常皮肤组织比较,膜联蛋白A2在瘢痕疙瘩与增生性瘢痕中表达均增高,差异有统计学意义(P<0.05);与增生性瘢痕比较,膜联蛋白A2在瘢痕疙瘩中表达增高,差异有统计学意义(P<0.05)。结论膜联蛋白A2可能参与了瘢痕疙瘩和增生性瘢痕的发生发展,同时膜联蛋白A2在瘢痕疙瘩的浸润性生长中可能起到一定的作用。
Objective To investigate the expression of Annexin A2 in keloids, hypertrophic scars and normal skins, then to discuss the mechanism and significance of Annexin A2 in keloids. Methods The expression of Annexin A2 were examined in 25 cases of keloids, hypertrophic scars and 10 cases of normal skins by immunohisto- chemistry, Western blot and fluorescent quantitative PCR respectively. Then the differentiation of the expres- sion in keoids, hypertrophic scars and normal skins were analyzed quantitatively and qualitatively by means of the image analysis system. Results The results of immunohistochemistry, Western blot and fluorescent quan- titative PCR showed：the differences were statistically significant in keloids and hypertrophic scars compared with normal skins （P 〈 0.05）. The expression of Annexin A2 in keloids was higher than that in hypertrophic scars, there was statistical difference between them （ P 〈 0.05 ）. Conclusion Annexin A2 may play an im- portant role in the development of keloids and hypertrophic scars. The invasive growth of keloids may need Annexin A2.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2013年第11期1088-1091,1116,共5页
The Chinese Journal of Dermatovenereology
基金
国家自然科学基金(81041076)
江苏省"六大人才高峰"第七批项目(2010-WS039)
