摘要
建立了快速灵敏测定皮克级（R）5．（2-氨基丙基）-2-甲氧基苯磺酰胺（月型手性胺）的流动注射．化学发光法。以R型手性胺对鲁米诺．牛血清白蛋白化学发光强度显著的抑制作用为基础，建立了化学发光强度降低值与尺型手性胺浓度对数值的线性关系，线性范围为0．03—70ng／mL，检出限为10pg／mL（3叮）。本方法成功用于盐酸坦索罗辛缓释胶囊及人体唾液、尿液及血清中冗型手性胺的测定，回收率为96．0％-104．2％，相对标准偏差小于4．0％（n=7）。
An ultrasensitive and rapid flow injection-chemiluminescence method for the determination of （R） -5-（2-amino-propyl） -2-methoxy-benzenesulfonamide （ R-APMB ） at pictogram level in pharmaceuti- cal preparations and biological fluids is established, based on the inhibitory effect of R-APMB on luminol bull serum albumin CL reaction in the flow injection system. The decrement of CL intensity is linearly pro- p？rtional to the logarithm of R-APMB concentration ranging from 0.03 to 70 ng/mL, with the limit of de- tection of 10 pg/mL （3or）. This method has been successfully applied to the determination of R-APMB in tamsulosin hydrochloride sustained release capsules and human saliva, urine and serum samples with the recoveries of 96.0% - 104.2% and the RSD of less than 4.0% （ n = 7）.
出处
《应用化工》
CAS
CSCD
2013年第11期2091-2093,2096,共4页
Applied Chemical Industry
基金
国家自然科学基金资助项目(20975081)
西安医学院大学生科研计划项目(12DXS04)
