摘要
观察联合应用siRNA对HepG2.2.15细胞中HBV抗原表达和复制的抑制作用。应用ELISA方法检测HBeAg和HBsAg;HBV DNA水平用实时定量PCR测定;用RT-PCR检测HBV mRNA水平。结果显示,实验中应用的HBV特异性siRNA均具有明显的抗HBV抗原表达和病毒复制作用;联合应用siRNA较单独应用具有更强的抗HBV作用。可见,HepG2.2.15细胞中联合应用siRNA对HBV复制的抑制作用比单独应用siRNA更有效。
Joint application of small interfering （siRNA） to inhibit the expression and replication of hepatitis B virus （HBV） antigen in HepG2.2.15 cells was observed using HBeAg testing with ELISA, HBV DNA level real time quantitative PCR testing, and HBV mRNA level testing with reverse-transcript PCR. The results showed that the HBV specific siRNAs used in the experiments possessed remarkable anti-HBV effects on HBV antigen expression and virus replication, and joint application of the siRNAs had stronger anti-HBV effects than single application of the siRNA. Therefore, joint application of siRNAs in HepG2.2.15 cells was more effective on the inhibition of HBV replication than single application of siRNA.
出处
《微生物学杂志》
CAS
CSCD
2013年第5期50-52,共3页
Journal of Microbiology
基金
黑龙江省教育厅基金(12511276)
