摘要
本研究用无特定病原体 (SPF)鸡胚增殖禽流感病毒鹅体分离株A/Goose/Guangdong/1/96 (H5N1) (GD1/96 ) ,提取病毒基因组总RNA ,运用反转录_聚合酶链反应 (RT_PCR)技术分别扩增该病毒分离株的 8个基因片段的cDNA ,分别克隆后进行序列测定。序列分析结果表明 ,所扩增到的 8个片段均包含相应的病毒基因的完整开放阅读框架。GD1/96株与 1997年香港禽流感事件中的 4株香港流感病毒分离株 (分别来自人、鸡、鸭和鹅 )的HA基因的高度同源性说明它们可能起源于同一种系 ,但NS基因的差异说明它们分属于不同的基因群系。GD1/96株与香港流感病毒分离株的核苷酸和推导的氨基酸序列的同源性较低 ( 6 3.9%～ 98.1%) ,而香港流感病毒分离株之间的核苷酸和氨基酸序列的同源性很高 ( 96 .5 %～ 10 0 %) ,且香港各流感病毒分离株的NA均缺失 5 7个核苷酸 ( 19个氨基酸 )残基。因此 ,内地H5N1分离株GD1/96与
In this report, an avian influenza virus (AIV) isolate A/Goose/Guangdong/1/96(H5N1) (GD1/96) was propagated in specific pathogen free chicken embryo. The viral RNAs were extracted and used in reverse transcription-polymerase chain reaction for amplifying the eight full-length cDNAs of the virus genes. The cDNAs were then cloned at Sma I site of pUC119 and sequenced. The results show that each of 8 gene cDNA fragments contains the whole open reading frame of each gene. High homology exists among the hemagglutinins (HAs) of GD1/96 and other 4 influenza virus isolated from human, chicken, duck and goose respectively during 1997 Hong Kong Avian Flu which implys that all the viruses can be classified into Eurasian lineage of H5 subtype AIVs. However, very low homology associated with the non-structural (NS) proteins between GD1/96 and the Hong Kong flu viruses indicates that GD1/96 comes from different genetic groups. Furthermore, lower homology (63.9%～98.1%) of the entire genes exists between GD1/96 and the Hong Kong isolates when compared to the homology (96.5%～100%) among the Hong Kong viruses. In addition, deletion of 57 nucleotides (or 19 amino acids) occurred within the neuraminidase molecule of all Hong Kong viruses instead of GD1/96. The data above suggests that GD1/96 is not the pathogen and even not the gene donor of the Hong Kong viruses in “1997 Hong Kong Avian Flu”.
出处
《中国预防兽医学报》
CAS
CSCD
2000年第3期232-235,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
国家自然科学基金资助项目! ( 3980 0 112 )
"973"项目资助! (G19990 1190 2
G19990 1190 5 )
