摘要
目的:比较补中益气汤不同配伍对脾虚发热证大鼠甲状腺和免疫功能的影响。方法:雄性大鼠随机分为对照组、脾虚发热模型组、补中益气汤原方组、补中益气去柴升组、补中益气大剂柴升组、单纯大剂柴升组共6组。采用饮食失节+过度疲劳+注射LPS(80μg/kg)法复制脾虚发热大鼠模型。于第18d各给药组给予相应的中药(补中益气汤原方组、补中益气去柴升组、补中益气大剂柴升组、单纯大剂柴升组四组大鼠分别按剂量6.83g/kg、6.30g/kg、8.70g/kg、2.52g/kg)灌胃,连续5d。第22d断头取血检测IL-1β、IL-2、IL-6、T3、T4、TSH。结果:与对照组比较,脾虚发热模型组大鼠的血T3、T4、TSH、IL-1β、IL-2降低,IL-6升高(P<0.05)。与脾虚发热模型组比较,补中益气汤原方组(6.83g/kg)、补中益气去柴升组(6.30g/kg)、补中益气大剂柴升组(8.70g/kg)大鼠的血T3、T4、TSH、IL-1β和IL-2均明显升高,IL-6下降(P<0.05或P<0.01);单纯大剂柴升组(2.52g/kg)大鼠的血T3、T4、TSH和IL-1β、IL-2、IL-6均无明显变化。结论:脾虚发热模型大鼠存在免疫和甲状腺轴的功能异常;补中益气汤对脾虚发热大鼠的免疫和甲状腺轴异常均有改善作用,减去升麻、柴胡或增加其用量均使其改善作用减弱,单纯大剂升麻与柴胡配伍无改善作用,补中益气汤的效用与方中升麻、柴胡及其剂量的选配有关。
To compare the Effect of Buzhongyiqi Tang and different compatibilities on thyroid and immune function of spleen-qi defi ciency with fever syndrome. Methods： The rats were randomly assigned into the normal group, spleen-deficiency with fever group, Buzhongy iqi Tang group, Buzhongyiqi Tang without Chaihu Shengma（ 柴胡,升麻） group, Buzhongyiqi Tang with large dosage Chaihu Shengma（柴胡,升麻） group and large dosage Chaihu Shengma group, 10 rats in every group. The spleen-qi deficiency with fever model was established by excess fatigue ＋ out of constant diet ＋ LPS injection. From the 18th day, each group rats were fed with medicine for 5 days. At the 22th day, the IL-β, IL-2, IL-6, T3, T4, TSH were measured after LPS injection. Result： The IL-Iβ,IL-2, I3, T4, TSH of the spleen-qi defi ciency with fever group had obviously decreased and IL-6 increased （ P 〈 0.05）. Compared with the spleen-qi deficiency with fever group, the T3, T4, TSH IL-1β, IL-2 of Buzhongyiqi Tang group, Buzhongyiqi Tang without Chaihu Shengma group and Buzhongyiqi Tang with large dosage Chaihu Shengma group had obviously increased and IL-6 decreased （ P 〈 0.05 或 P 〈 0.01 ） ; the T3,T4, TSH IL-1β, IL-2 of large dosage Chaihu Shengma group had no obvious changes （ P 〉 0.05）. Conclusion： The thyroid and immune function of spleen-qi deficiency with fever syndrome are abnormal. Buzhongyiqi Tang（6.83g/kg）has great effect on the thyroid and immune function of spleen-qi deficiency with fever model and its effect will be subdued when without Chaihu Shengma（6.30g/kg） or with large dosage of Chaihu Shengma（8.70g/ kg）. Compatibility of large dosage Chaihu and Shengma （2.52g/kg） has no above effect. The effectiveness of Buzhongyiqi Tang is connected with Chaihu Shengma and their dosage.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2013年第5期21-23,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金资助项目(81173193)
关键词
补中益气汤
脾虚发热
配伍
甲状腺
免疫
Buzhongyiqi Tang （ 补中益气汤）
compatibility
thyroid
immunity
