摘要
目的:观察复方紫黄乳膏对大鼠皮肤深Ⅱ度烧伤的愈合作用。方法:利用混合燃料法制备大鼠背部皮肤深Ⅱ度烧伤模型,将大鼠随机分为模型组、紫黄乳膏高、低剂量组和美宝组,每组12只。紫黄乳膏高、低剂量组和美宝组的大鼠每天1次分别涂以复方紫黄乳膏(0.3 g/g,0.15 g/g)和美宝湿润烧伤膏,连续给药28 d,采用Nagelschmidt法计算创面愈合率,ELISA检测烧伤后大鼠血清中肿瘤坏死因子(TNF-α)、表皮生长因子(EGF)和白介素-6(IL-6)的表达水平,并于烧伤后的14 d和28 d对大鼠创面皮肤作病理学检查。结果:与模型组相比,7 d、14 d、28 d紫黄乳膏高、低剂量组和美宝组的创面愈合率明显升高;与模型组和美宝组相比,紫黄乳膏高、低剂量组的平均愈合时间明显缩短;紫黄乳膏高、低剂量组和美宝组大鼠血清中TNF-α和IL-6水平恢复较模型组快,EGF含量也较模型组高。结论:复方紫黄乳膏对大鼠皮肤深Ⅱ度烧伤有显著的治疗作用。
To observe the therapeutical effect of Fufang Zihuang cream on wound healing in rats with deep degree Ⅱ burning. Meth ods： Rat models with deep degree Ⅱ burning were established with mixed fuel. The rats were randomly divided into model group, high, low - dose Zihuang cream group and Meibao group with 12 rats in each. The rats in high, low - dose Zihuang cream group and Meibao group were treated respectively with Fufang Zihuang cream （0.3 g/g, 0.15 g/g） and Meibac Shirun Shaoshang Cream for 28 days. The wound healing rate was calculated by Nagelschmidtg method. ELISA was used to detect the TNF-α ,EGF and IL-6 in rat plasma, and the pathologic examine was carried out on day 14 and 28 after injury. Results： The wound healing rates of high, low - dose Zihuang cream group and Meibao group on day 7, 14 and 28 were higher than those of model group （ P 〈 0.05 or P 〈 0.01 ）. The healing time on average of high, low - dose Zi huang cream group was shorter than that of Meibao group or＇model group （P 〈 0.05 or P 〈 0.01 ）. Meanwhile, the contents of TNF-α and IL-6 in rat plasma of high, low - dose Zihuang cream group and Meibao group got back to normal earlier and the content of EGF was higher. Conclusion： Fufang Zihuang Cream has conspicuous therapeutical effect on wound healing in rats with deep degree Ⅱ burning.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2013年第5期132-134,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
南京军区医学科研课题(NO 11MA012)
关键词
复方紫黄乳膏
烧伤治疗
致炎细胞因子
Fufans Zihuang cream（复方紫黄乳膏）
Meibao Shirun Shaoshang Cream（美宝温润烧伤膏）
bum treatment
proinflamma tory cytokine
