摘要
[Objective] This study aimed to compare the contents of total alkaloids in different solvent extracts from climbing groundsel herb produced in Guizhou Province and their antibacterial activity. [Method] The content of total alkaloids was measured by UV spectrophotometry, and Oxford cups were used to investigate the antibacterial activity of each solvent extract. [Result] There was a good linear correlation between the absorbance measured by the UV spectrophotometer and the content of total al- kaloids within the concentration of 0.011 0-0.054 8 mg/ml at 207 nm, and the re- gression equation was Y=23.654X＋0.021, R=0. 999 7 and the average recovery rate was 99.2%. The contents of total alkaloids in 60% ethanol extract, 95% ethanol and water ethanol were 38.71, 52.25 and 60.50 mg/g, respectively. The 60% ethanol ex- tract had strong antibacterial activity against Streptococcus pneumoniae, Staphylo- coccus aureus and Escherichia coil; the water extractive had stronger antibacterial activity against Escherichia colr, 95% ethanol extract had weak antibacterial activity, with no inhibition of Pseudomonas aeruginosa. [Conclusion] This method is accurate, simple, with good repeatability, which can be used for the determination of alkaloids content of the climbing groundsel herb; there is no positive correlation between the content of total alkaloids in different solvent extracts of climbing groundsel herb and their antibacterial activity.
试图从爬杂草植物在不同溶剂摘录比较全部的碱的内容的这研究在贵州生产了省和他们的抗菌剂活动。全部的碱的内容被紫外 spectrophotometry 测量，并且牛津杯被用来调查每篇溶剂摘录的抗菌剂活动。在紫外分光光度计和全部的碱的内容在 207 nm 在 0.0110-0.0548 mg/ml 的集中以内测量的吸收度之间有好线性关联，并且回归方程是 Y=23.654X+0.021， R=0.9997 和平均恢复率是 99.2% 。在 60% 乙醇摘录， 95% 乙醇和水乙醇的全部的碱的内容分别地是 38.71， 52.25 和 60.50 mg/g。60% 乙醇摘录对链球菌 pneumoniae，葡萄球菌 aureus 和 Escherichia coli 举办了强壮的抗菌剂活动；水对 Escherichia coli 的引得出的有的更强壮的抗菌剂活动；95% 乙醇摘录举办了弱抗菌剂活动，没有 Pseudomonas aesvginosa 的抑制。
基金
Supported by the Clinical Scientific Research Capacity Construction Program for Country-level Hospitals of Department of Health,Guizhou Province(gzwxky2011-1-022)~~
