摘要
以长白山槭叶草(Mukdenia rossii(Oliv)Koidz)1a生种苗为试材,研究了不同浓度(0%(CK)、10%、15%、20%、25%)和不同时间(24、48、72、96h)聚乙二醇6000(PEG-6000)模拟干旱胁迫对槭叶草叶片生理生化指标的影响及其耐旱性,以期为槭叶草在园林绿化中的应用提供理论依据。结果表明:槭叶草可通过渗透调节作用适应轻度干旱胁迫,表现出一定的耐旱性。随胁迫强度的增加和胁迫时间的延长槭叶草叶片相对含水量(RWC)呈下降趋势,相对电导率(REC)呈上升趋势,丙二醛(MDA)含量大体呈上升-下降-上升的趋势;脯氨酸(Pro)含量呈先升后降的趋势;SOD活性呈上升-下降-上升-下降的趋势,均与对照差异显著(P<0.05)。相关性分析表明,REC、MDA含量、Pro含量与RWC呈显著性相关,SOD活性与RWC无显著性相关。RWC、REC、MDA含量、Pro含量可作为评价槭叶草耐旱性的依据。
Taking the annual seeding of Mukdenia rossii （Olive） Koidz as experimental material, the method of stimulated drought stress by applying PEG-6000 were adopted,the effect of different concentrations of PEG-6000 （be 0% （CK）, 10%,15;,20%,25 % respectively） and different time（24,48,72,96 h） on the physiological and biochemical indexes of Mukdenia rossii and its drought tolerance were studied,thus provided a theoretical basis for the application of Mukdenia rossii in landscaping. The results showed that the Mukdenia rossii could adapt to mild drought stress through osmo- regulation thus showed drought resistance to some extent. Along with the increasing of the stress intensity and the extension time,several changes occurred. The relative water content （RWC）of Mukdenia rossii was in downward trend while the relative electrolytic conductivity （REC） was in upward trend. The content of malonaldehyde （MDA） was in a trend of first rising,then falling and bakes to rising again. The proline （Pro） content was in trend of rising after the first drop. The SOD activity had a trend of up-down-up-down. All of them showed obvious difference in comparing （P〈 0. 05）. The related analysis indicated that there was a significant correlation between REC, MDA content, Pro content and RWC. On the contrary,that relation didn＇t occur between SOD activity and RWC. Therefore,RWC,REC,MDA content and Pro content could be used to evaluate the drought tolerance of Mukdenia rossii.
出处
《北方园艺》
CAS
北大核心
2013年第22期74-78,共5页
Northern Horticulture
基金
吉林省科技厅科技支撑计划资助项目(20100259)
关键词
槭叶草PEG
干旱胁迫
生理响应
Mukdenia rossii （Olive） Koidz
PEG-6000
drought st ress
physiological responses
