摘要
目的了解宁波地区急性腹泻患者中副溶血弧菌（Vibrio parahaemolyticus，VP）分布及分子分型特征。方法对2012年4～10月宁波地区VP临床分离株进行血清型分型；用PCR检测毒力基因tlh、tdh和trh；对所有菌株进行多位点序列分型（MLST）分析。结果962份急性腹泻患者粪便标本中71份VP阳性，分离率为7．38％；71株菌分属17种血清型，O3：K6是最主要的血清型，共44株（61．97％），其次是04：K8（5株）；毒力基因检测发现71株tlh均呈阳性（100％），70株tdh阳性98．59％，仅3株trh阳性。MLST分析发现13种序列型（STs），主要为$33型45株，占63．38％。结论宁波地区急性腹泻患者中VP分离株以O3：K6血清型为主，ST3型为优势序列型。
Objective To understand the distribution and molecular characteristics of Vibrio parahaemolyticus in the patients with acute diarrhea of Ningbo district for prevention and control of V. parahaemolyticus infection. Methods The feces samples were collected from the patients with acute diarrhea during April to October, 2012. The isolates of V. parahaemolyticus were detected for serotyping and identifying the virulence genes, tlh, tdh and tdh. Molecular characteristics were analyzed by using multi-locus sequence typing （MLST）. Results Among the 962 feces samples 71 （7.38%） were V. parahaemolyticus-positive. The 71 strains belonged to 17 dif- ferent serotypes. O3：K6 was the predominant serotype accounting for 61.97% （44 strains）, followed by O： K8 （5 strains）. Tlh gene was presented in all the examined strains and tdh gene was positive in 70 strains （98.59%）. Only three strains were trh-positive. MLST analysis showed 13 sequence types （STs） were foundin the 71 V. parahaemolyticus strains, and ST3 was the predominant type accounted for 63.38% （45 strains）. Conclusion O3：K6 serotype was the most prevalent type of V. parahaemolyticus in Ningbo dis- trict. ST3 was the master sequence type.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2013年第10期792-794,共3页
Chinese Journal of Clinical Laboratory Science
基金
"十二五"国家科技重大专项(2012ZX10004-210)
关键词
副溶血弧菌
血清型
毒力
多位点序列分型
Vibrio parahaemolyticus
serotype
virulence
multi-locus sequence typing
