摘要
目的:测定关节熏洗汤剂中升麻素苷和5-O-甲基维斯阿米醇苷含量。方法:采用高效液相色谱法。色谱柱:Agilent Zorbax SB-C_(18)柱(250 mm×4.6 mm,5μm);流动相:甲醇-乙腈-水(12:10:78);检测波长:254 nm;流速:1 ml·min^(-1);柱温:30℃。结果:升麻素苷和5-O-甲基维斯阿米醇苷的线性范围分别为0.035~0.350μg和0.037~0.370μg(r分别为0.999 9和0.999 8),平均回收率分别为101.49%(RSD=2.5%)、98.77%(RSD=2.2%)。结论:升麻素苷和5-O-甲基维斯阿米醇苷的含量测定为关节熏洗汤剂的疗效提供了初步依据。
Objective： To determine the contents of prim-O-glucosylcimifugin and 5-O-methylvisammioside in Guanjie Xunxi de- coctions. Method： An Agilent Zorbax SB-CI8 （250 mm ~ 4.6 mm,5 txm） was used with methanol-acetonitrile-water （ 12： 10： 78） as the mobile phase, the detection wavelength was 254 nm, the flow rate was 1.0 ml min- 1 and the column temperature was 30C. Re- sult： The linear range of prim-O-glueosylcimifugin and 5-O-methylvisammioside was 0. 035-0.350 μg （ r = 0. 999 9 ） and 0.037- 0.370 μg（ r = 0. 999 8）, respectively, the average recovery was 101.49% （RSD = 2.5% ） and 98.77% （ RSD = 2.2% ）, respective- ly. Conclusion： The determination of prim-O-glucosylcimifugin and 5-O-methylvisammioside provides the preliminary basis for the study on the efficacy of Guanjie Xunxi decoctions.
出处
《中国药师》
CAS
2013年第11期1639-1641,共3页
China Pharmacist
