摘要
采用同源基因克隆和RLM-RACE法,从牡丹品种‘凤丹’花器官中克隆了PsAP2,其cDNA全长2 138 bp,包含1个长为1 533 bp的开放阅读框编码510个氨基酸。BlastX比对发现,PsAP2与许多植物的APETALA2蛋白同源性高达70%以上;与芍药、葡萄、可可树和杨树的相似率分别为97%、72%、68%和63%。基因结构分析发现,PsAP2编码区基因组片段为2 477 bp,含有10个外显子和9个内含子,均符合GT-AG法则。亚细胞定位表明,PsAP2定位于细胞核中,暗示其发挥转录因子的功能。PsAP2的获得将为后续功能分析提供基础,为牡丹花型分子育种提供候选基因。
A full-length cDNA was isolated from flower of tree peony （ Paeonia suffruticosa Andr. ） ‘ Fengdan＇ , named as PsAP2, using the degenerated RT-PCR and RLM-RACE. PsAP2 cDNA was 2 138 bp in length, harboring an open read-ing frame of 1 533 bp coding 510 amino acids. Sequence alignment analysis showed that the protein sequence encoded by PsAP2 was highly conserved in planta with an maximum identity up to 70%. The amino acids encoded by PsAP2 were high-ly homologous to the APETALA2 genes of Paeonia lactiflora, Vitis vinifera, Theobroma cacao and Populus tomentosa with homology of 97%, 72% , 68% and 63% , respectively. Gene structure analysis indicated that the genomic DNA of PsAP2 coding sequence was 2 477 bp, including 10 exons and 9 introns, which were obey to the GT-AG rule. Subcellular locali-zation demonstrated that PsAP2 was present in nuclear, suggesting its role as a transcription factor. The isolated PsAP2 gene will lay an important foundation for functionally analyzing and provide candidate gene for molecular breeding of tree pony.
出处
《林业科技开发》
北大核心
2013年第6期26-30,共5页
China Forestry Science and Technology
基金
上海市绿化和市容管理局2011年科学技术项目辰山专项(编号:JB110325)
关键词
牡丹
花发育
AP2
转录因子
Paeonia suffruticosa Andr
flower development
AP2
transcription factor
