摘要
目的:优选姜椒凝胶剂的处方工艺并探讨其体外经皮渗透性。方法:以辣椒素24 h单位面积累积渗透量为评价指标,通过L9(34)正交试验考察甘油、丙二醇、氮酮用量对姜椒凝胶处方工艺的影响;以大鼠离体皮肤为屏障,运用Franz扩散池作为体外经皮释药模型,考察姜椒凝胶的体外透皮特性。采用HPLC测定辣椒素含量,色谱条件为Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.1%磷酸水(65∶35),流速1.0 mL·min-1,检测波长280 nm,柱温40℃,进样量20μL。结果:姜椒凝胶处方的最佳组合为2%卡波姆,10%甘油,20%丙二醇和1%氮酮;其体外经皮渗透符合零级动力学方程,经皮渗透速率0.353μg·h-1·cm-2。结论:该处方设计合理,适宜皮肤局部用药。
Objective： To optimize formulation of Jiangjiao gels and investigate its in vitro percutaneons penetration. Method： Taking cumulative permeation amount per unit area in 24 h of capsaicin as index,effect of dosages of glycerol,propylene glycol and azone on formulation process of capsaicin was investigated by orthogonal test;With isolated rat skin as transdermal barrier,Franz diffusion cell was used as in vitro percutaneous release model,then in vitro transdermal permeability of capsaicin was investigated.The content of capsaicin was determined by HPLC,chromatographic conditions were as follows：Diamonsil C18 column（4.6 mm250 mm,5 μm）,mobile phase of methanol-0.1% phosphoric acid solution（65：35）,flow rate 1.0 mL min-1,detection wavelength 280 nm,column temperature 40℃,injection volume 20 μL. Result： Optimal formulation of Jiangjiao gels was 2% carbomer,10% glycerol,20% propylene glycol and 1% azone;In vitro transdermal permeation of capsaicin in Jiangjiao gels fitted zero order kinetics equation,percutaneous permeation rate was 0.353 μg h-1 cm-2. Conclusion： Optimized formulation was reasonable and suitable for topical skin.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第23期28-31,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
重庆市教委自然科学基金项目(KJ121801)
关键词
姜椒凝胶
辣椒素
经皮渗透
正交试验
处方工艺
单位面积累积渗透量
Jiangjiao gels
capsaicin
percutaneous penetration
orthogonal test
formulation process
cumulative permeation amount per unit area
