摘要
目的:研究唑来膦酸(ZOL)对破骨细胞(OC)生成的影响,探讨Atp6v0d2基因在其中发挥的作用。方法:应用小鼠颅骨成骨细胞(OB)与小鼠单核巨噬细胞RAW264.7建立共培养体系。细胞分为对照组和ZOL处理组(ZOL处理24 h)。在不同时间点收获细胞,检测Atp6v0d2基因表达、OC生成和骨吸收情况。结果:ZOL组TRAP染色阳性多核OC和骨吸收陷窝显著少于对照组(P<0.01);Atp6v0d2基因表达在ZOL组显著下降(P<0.01)。结论:ZOL可显著抑制OB与RAW264.7共培养体系中OC生成和骨吸收功能,下调Atp6v0d2基因表达。
Objective：To study the effect of zoledronate（ZOL） on Atp6v0d2 gene expression and osteoclastogenesis.Methods：Mouse calvarial osteoblasts（OB） and mouse mononuclear phagocyte RAW264.7 cells were used to establish a co-culture system.In ZOL group the cells were treated by ZOL for 24 h,while in control group the cells were cultured without ZOL.The cells were harvested at different time points and gene expression of Atp6v0d2,osteoclastogenesis and bone resorption were examined.Results：The number and size of TRAP positive multi-nucleated cells and absorption lacunae were all significantly decreased in ZOL group when compared with the controls （P ＜ 0.01）.Atp6v0d2 gene expression was down-regulated in ZOL group （P ＜ 0.01）.Conclusion：In the OB-RAW264.7 co-culture system,ZOL may inhibit Atp6v0d2 expression and osteoclast formation.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2013年第6期766-769,共4页
Journal of Practical Stomatology
基金
国家自然科学基金(编号:81270965)
河北省自然科学基金(编号:C2011401044)
