摘要
[目的]探讨D-四氢掌叶防己碱在HepG2肝癌细胞中对脂肪蓄积的抑制作用及相关机制.[方法]用D-四氢掌叶防己碱(0,5,10,20,40,80μmo/L)处理HepG2肝癌细胞24 h,采用MTS法测定细胞毒性;采用Western blot方法检测与脂肪积蓄有关的AMPK蛋白质的表达情况;采用RT-PCR方法检测与AMPK信号通路有关的SREBP1c、脂肪酸合酶(FAS)及硬脂酰辅酶去饱和酶(SCD1)等相关基因的表达情况.[结果]D-四氢掌叶防己碱呈浓度依赖性地抑制三酰甘油的含量,增加AMPK磷酸化;D-四氢掌叶防己碱抑制与脂肪合成有关的SREBP1c、FAS及SCD1基因的表达.[结论]D-四氢掌叶防己碱在HepG2肝癌细胞中通过AMPK信号通路抑制脂肪蓄积.
OBJECTIVE To study the inhibitory effects and its related mechanism of D-tetrahydropalmatine on lipid accumulation in HepG2 cells. METHODS HepG2 cells were treated with D-tetrahydropalmatine （0, 5, 10, 20 and 40 μmol/L） for 24 h. The cell toxicity of the HepG2 cells were detected by MTS, and the expressions of AMPK was mesured by Western blot, and the expressions of signaling pathway related genes such as SREBPlc, FAS and SCD1 were detected by RT-PCR. RESULTS D-tetrahydropalmatine dose-dependently inhibited the contents of triglyceride, and increased the phosphorylations of AMPK, and inhibited the expressions of lipogenesis gene （SREBPlc, FAS, SCD1）. CONCLUSION D-tetrahydropalmatine inhibits hepatic lipid accumulation in HepG2 human hepatoma cells via AMPK signal pathway.
出处
《延边大学医学学报》
CAS
2013年第3期174-177,共4页
Journal of Medical Science Yanbian University
基金
延边大学学校项目
编号:延大科合字(2012)第32号
吉林省中医药管理局项目
编号:吉中医药发2012-156
