摘要
三聚氰胺与胸腺嘧啶(T)之间能够通过三个氢键结合,以富T的DNA探针为识别元件,结合DNA修饰的纳米金颗粒放大技术,以电活性物质钌胺作为信号分子,发展了一种高灵敏检测三聚氰胺的电化学传感器,该传感器具有良好的特异性和灵敏度,检测下限低至0.5 nmol/L。
Melamine could form triple H-bonds with thymine in aqueous medium. An electrochemical biosensor for melamine detection based on DNA modified gold nanoparticles amplification strategies was demonstrated. In this system, the T-rich DNA probes were immobilized on electrode surface as recognition element. The cationic electroactive complex [Ru（NH3）6]3＋ was bound to the DNA via electrostatic interactions as signal molecules. Since hundreds of DNA molecules were loaded on single gold nanopartiele, a good specificity and sensitivity was obtained in this method, the detection limit was ca. 0.5 nmol/L.
出处
《化学传感器》
CAS
2013年第3期17-21,共5页
Chemical Sensors
