摘要
目的 研究动脉硬化性脑梗死 (ACI)患者中的载脂蛋白E(ApoE)基因的分布 方法 利用聚合酶链式反应 (PCR)技术扩增ApoE基因含编码 112位和 15 8位氨基酸的片段 ,并用限制性片段长度多态性 (RFLP)技术对ACI患者和相应健康对照的ApoE基因进行分型 ,从而进行ACI与ApoE等位基因多态性的关联分析 .结果 ACI患者中ApoE等位基因ε4占 2 8.30 % ,明显高于正常人对照组的 7.6 4% ,而等位基因ε3则占 5 7.5 5 % ,低于正常人对照组的84.12 % ,均有极显著性差异 (p<0 .0 1) .结论 PCR -RFLP是一种快速有效的ApoE基因分型方法 ,ε4可能为ACI的易感因子 ,而ε3则为保护因子 .
Objective To study the distribution of ApoE gene in patients with ACI.Methods ApoE gene fragment containing amino acid positions 112 and 158 was amplified by the polymerase chain reaction (PCR),and the restriction fragment length polymorphism(RFLP) technique was used to classify the ApoE gene of patients with ACI and age-matched controls,and to analyse the relation of ACI and ApoE allele.Results The prevalence of ApoE ε4 allele was 28.30% in ACI,that was significantly higher than 7.64 % in control,but ε3 allele in ACI was 57.55 %,that was significantly lower than 84.12% in control,both of them were significant difference(p<0.01).Conclusions PCR-RFLP technique was a rapid and effective method in classifing ApoE gene ,ε4 allele seemd to be a genetic susceptible factor in ACI,but ε3 allele probable was a protecture factor in ACI.
出处
《现代临床医学生物工程学杂志》
2000年第4期250-251,共2页
Journal of Modern Clinical Medical Bioengineering
