摘要
目的:建立高效液相色谱法测定前列安通胶囊中芍药苷和盐酸小檗碱含量的方法。方法:采用ZORBAX SB-C_(18)色谱柱,流动相为乙腈-0.05 mol·L^(-1)磷酸二氢钾溶液(磷酸调pH值至3.0)(16:84)(A)-乙腈(B)采用梯度洗脱(0~10 min,A 100%;10~20 min,A 100%~90%;20~30 min,A 90%~85%;30~35 min,A 85%);检测波长:230 nm;流速:1.0 mL·min^(-1)。结果:芍药苷在7.7~192μg·mL^(-1)线性关系良好(r=0.999 9),平均回收率为99.6%,RSD=1.1%;盐酸小檗碱在7.2~180μg·mL^(-1)线性关系良好(r=0.999 9),平均回收率为99.8%,RSD=0.5%。结论:实验结果准确,重现性好,可用于前列安通胶囊的质量控制。
Objective： To establish HPLC method for determination of paeoniflorin and berberine hydrochlo-ride in Qianlieantong capsules. Methods： A stable HPLC method was established and the chromatography was accom-plished on a ZORBAX SB-C1s column, the mobile phase consisted of acetonitrile-0. 05mol·L^-1 dipotassium hydrogen phosphate solution（ adjusted pH to 3.0 with phosphoric acid） （ 16： 84） （A） - acetonitrile（B） in a gradient mode）0-10 min, A100% ; 10-20 min, A100%-90% ; 20-30 min, A90% - 85% ; 30 - 35min, A85% ）, the detection wavelength was 230 nm and the flow rate was 1.0 mL· min^ - 1. Results： The calibration curve of paeoniflorin was linear within a range of 7.7-192 μg·mL^-1（r = 0. 999 9）, and the average recovery was 99. 6% with a corresponding RSD of 1.1% ; The calibration curve of berberine hydrochloride was linear in range 7.2-180 μg·mL^-1（r =0. 999 9）, and the average recovery was 99. 8%, with a corresponding RSD of 0. 5%. Conclusion： The method is accurate and re-producible, which can be used for the quality control of Qianlieantong capsules.
出处
《中国现代中药》
CAS
2013年第11期992-994,共3页
Modern Chinese Medicine
