摘要
以苦丁冬青苦丁茶为研究材料，通过柱层析和半制备色谱分离纯化得到6种咖啡酰奎尼酸（CQA）类物质产品，并采用TSKgelODS-80TsQA色谱柱和二极管阵列检测器（DAD），建立一种便捷、灵敏的分析苦丁冬青苦丁茶中cOA类物质的高效液相色谱分析方法。结果表明，通过半制备色谱可分离得到纯度95％以上的6种CQA类单体物质：3-CQA、4-CQA、5-CQA、3,4-diCQA、3，5-diCQA和4，5-diCQA。色谱条件：TSKgelODS-80TsQA色谱柱，流动相为水．甲醇-5％。甲酸体系，梯度洗脱，流速0．5mL／min，柱温40℃，检测波长326nm。该方法在一定范围内有良好线性，相关系数为0．9992-0．9999，平均加标回收率为96．34％，相对标准偏差均小于3％。
Six caffeoylquinic acid （CQA） derivatives were separated by column chromatography and semi-preparative high performance liquid chromatography （HPLC） from the crude extract of llex kudingcha C.J. Tseng. An HPLC method with DAD and TSKgel ODS-80 TsQA column was developed for the simultaneous determination of CQAs in Kuding- cha made from llex kudingcha C.J. Tseng. The results demonstrated that six CQA derivatives with 95% purity, 3-CQA, 4-CQA, 5-CQA, 3,4-diCQA, 3,5-diCQA and 4,5-diCQA, could be obtained through the purification of Ilex kudingcha C.J. Tseng by semi-preparative high performance liquid chromatography （HPLC）. The chromatographic conditions were determined as follows： oven temperature set at 40 ℃, gradient elution with methanol/formic acid （5%0） with a flow rate 0.5 mL/min, and detection at a wavelength of 326 nm. As results, good linearity was observed with correlation coefficients of 0.9992-0.9999, the average recovery of the CQA derivatives from spiked samples was 96.34%, with relative standard deviations （RSD） less than 3% （n = 3）.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第22期119-122,共4页
Food Science
基金
国家自然科学基金面上项目(31171666)
江苏高校优势学科建设工程资助项目
关键词
苦丁茶
苦丁冬青
咖啡酰奎尼酸
纯化
高效液相色谱
Kudingcha
Ilex kudingcha C.J. Tseng： caffeoylquinic acids
purification： high performance liquid chro-matography （HPLC）
