摘要
目的:从羊胎盘中分离纯化出具有免疫活性的小分子肽。方法:采用常规方法制备羊胎盘超滤液,利用反相高效液相色谱仪(RP-HPLC)对羊胎盘提取液进行分离纯化及纯度分析,并利用E-玫瑰花环实验对各组分进行免疫活性评价,利用Edman降解法对其组分进行N端测序。结果:利用RP-HPLC工艺成功分离出纯度较高的3种小分子肽,并确定其中两者具有免疫活性;对活性较强的肽进行测序、检索,结果表明为未知组分。结论:RP-HPLC是分离、分析活性肽类较理想的手段之一,并证实了羊胎盘含有具有免疫活性的小分子肽。
The purpose of this study was to isolate and purify small-molecule peptides with immunomodulating activ- ity from goat placenta. Ultrafiltration fraction from goat placenta was prepared by a conventional method, followed by isolation, purification and analysis by reversed phase-high performance liquid chromatography （RT-HPLC）. The im- munomodulating activity of its components was measured by E-rosette test and the N-terminal sequence was measured by Edman degradation reaction. Three small-molecule peptides with high purity were obtained, two of which were im- mune-active. The results of sequencing and retrieving showed that they were new peptides. RT-HPLC was one of the optimal methods to purify and analyze bioactive peptides. The small-molecule peptides from goat placenta revealed strong, immune activity.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第22期273-276,共4页
Food Science
关键词
羊胎盘小分子肽
反相高效液相色谱
E-玫瑰花环
goat placenta small-molecule peptides
reversed phase-high performance liquid chromatography （RT-HPLC）
E-rosette
