摘要
研究以残波烟草和斯托克通氏烟草为材料,采用直接观察和石蜡切片法对其大孢子发生和雌配子体发育过程进行了研究.结果表明2个种有很多相似之处:形态学上,均为管状花,自花授粉,果实为蒴果等;胚胎学上,二者均为二室子房,倒生多胚珠,大孢子母细胞经减数分裂形成直线型四分体,3个退化后剩下一个形成功能大孢子,成熟胚囊为八核七细胞胚囊.但2个种发育过程中也有不同之处:在形态和开花物候上,植株高度、花朵大小颜色等均有差异,从移栽至现蕾时间和花期长短也有差异;胚胎学上,珠被绒毡层退化时间、胚囊发育中细胞形态不同.2个种均未发现胚发育的异常及胚囊败育的现象.
The megasporogenesis Nicotiana stocktonii B. were studied and the development by applying paraffin of female gametophytes of Nicotianarepanda W. and section and morphological observation. The results showed that they were similar in morphology, they were self- pollination, tubiform floret, capsule. In embryology, they had bilocular ovaries, anatropous and multiple ovules, and after meiosis straight line tetrad was formed from megasporocytes, three of them degenerated,leaving only one as a functional megaspore to develope in embryo sac which had seven cells and eight nuclei. Difference was also shown both morphologically and embryologically be tween N. repanda and N. stocktonii as in plant heights, flower colors and sizes;they were also different in the time of forming buds , flowering time, degenerating time of tapetum and the shape of cells in the sac. The phenomenon of abnormalities and abortion of embryo sac were not found.
出处
《云南大学学报(自然科学版)》
CAS
CSCD
北大核心
2013年第6期866-873,共8页
Journal of Yunnan University(Natural Sciences Edition)
基金
云南省烟草公司项目(2011YN09)
云南大学生命科学学院植物科学研究所青年基金(ZW201002)
云南大学理(工)科校基金(2010BY007)
关键词
野生烟草
残波烟草
斯托克通氏烟草
雌配子体发育
大孢子发生
wild Nicotiana
Nicotiana repanda W.
Nicotiana stocktonii B.
development of female gametophyte
megasporogenesis