摘要
目的:研究小建中汤及其拆方对芍药苷含量的影响。方法:采用Diamonsil RP-C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-水为流动相,流速1.0 mL·min-1,检测波长230 nm。结果:小建中汤拆方前后芍药苷的含量有明显变化,其中去甘草组、去桂枝组、去饴糖组与全方组比较均有显著性差异(P<0.05);去生姜组、去大枣组与全方组比较无显著性差异(P>0.05)。结论:甘草和桂枝有助于白芍中芍药苷的溶出;大枣、生姜对白芍中芍药苷的溶出影响不大;饴糖可能会以小团块形式包裹芍药苷,使实验中芍药苷的萃取效率降低。
This study was aimed to investigate the effect of Xiao-Jian-Zhong decoction and its disassembled prescriptions on the content determination of paeoniflorin . A gradient HPLC system was used in the quantitative analysis of paeoniflorin on Diamonsil RP-C18 column （ 250 mm × 4 . 6 mm , 5 μm ） with the mixed solvents of acetonitrile and water . The flow rate was 1 . 0 mL？min-1 and the detection wavelength was 230 nm . The results showed that the content of paeoniflorin had great changes comparing with X iao-Jian-Zhong decoction with its different disassembled prescriptions . The analysis of variance showed that the difference among groups was sig-nificant （P 〈 0.05）. SNKq analysis showed that there was significant difference between groups 6 and 1, 6 and 2 , 6 and 5 （ P 〈 0 . 05 ） . But no significant difference was existed among group 6 , 3 and 4 （ P 〉 0 . 05 ） . It was concluded that liquorice and cassia twigs were helpful to the dissolution of paeoniflorin in the decoction . But jujube and ginger had few effects . The content of paeoniflorin in group 5 which does not include maltose was the highest among all groups . It may be due to the fact that paeoniflorin was wrapped in the maltose of X iao-Jian-Zhong decoction .
出处
《世界科学技术-中医药现代化》
北大核心
2013年第6期1338-1342,共5页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委面上项目(81173555):基于峻泻逐水及毒性与商陆皂苷甲变化相关的商陆炮制科学内涵的构建
负责人:张朔生
山西省卫生厅科技攻关项目(2011072):四逆汤抗心力衰竭作用物质基础研究
负责人:王瑞
关键词
小建中汤
拆方
芍药苷
含量测定
高效液相色谱法
Xiao-Jian-Zhong decoction, disassembled prescription, paeoniflorin, content determination, highperformance liquid chromatography
