摘要
目的研究纤溶酶原激活物抑制剂-1(plasminogen activator inhibitor 1,PAI-1)在大鼠胰腺组织中的表达,为临床上对重症急性胰腺炎(severe acute pancreatitis,SAP)患者使用抗凝剂及抗血小板药物治疗提供理论依据,并为进一步研究应用PAI-1抑制剂治疗胰腺炎提供形态学依据。方法应用免疫荧光组织化学双重标记技术,在激光共聚焦显微镜观察PAI-1在大鼠胰腺组织中的分布。结果 PAI-1免疫反应阳性细胞分布在SD大鼠胰腺的外分泌腺腺泡细胞,以及内分泌腺D细胞及PP细胞,阳性物质分布于细胞质。结论 PAI-1可能在胰腺起着非常重要的作用,为进一步研究应用PAI-1抑制剂治疗胰腺炎提供了形态学依据。
Objective To investigate the distribution of plasminogen activator inhibitor 1 （PAl-l） in the pancreas of normal SD rat to provide morphologic basis for further research on using PAI-1 inhibitor to cure acute pancreatitis. Methods The immunofluorescence histochemical double-labeling technique was used to observe the localization of PAI-1 in the pancreas of normal SD rats under a confocal laser-scanrfing microscope. Results PAI-1 immunoreactivity was found to be localized in the cytoplasm of the acinar cells, somatostatin-secreting cells（D cells）, and pancreatic polypeptide （PP）-secreting cells（PP cells）. Conclusion PAI-1 might play a critical role in the pancreas, which will provide morphologic basis for further research on using PAI-1 inhibitor to cure acute pancreatifis.
出处
《解剖科学进展》
CAS
2013年第6期506-508,共3页
Progress of Anatomical Sciences
基金
辽宁省科技计划项目(No.2009225022)
关键词
纤溶酶原激活物抑制剂-1
胰腺
大鼠
双重免疫荧光组织化学
plasminogen activator inhibitor 1
pancreas
rat
double-labeling immunofluorescence histochemistry
