摘要
关于马铃薯芽和花的杀虫活性研究,国内外未见文献报道。研究马铃薯无光干芽和花的杀虫活性及其对粘虫的作用方式,可为利用无光干芽和花创制新型植物源杀虫剂提供线索和理论依据。本研究采用虫体浸渍法和夹毒叶碟法测定了庄薯3号马铃薯无光干芽和花乙酸乙酯粗提物的杀虫活性。结果表明,庄薯3号马铃薯无光干芽和花乙酸乙酯粗提物对粘虫幼虫具有很强的触杀作用,稀释5倍液的校正死亡率分别为52.0%和61.36%。无光干芽和花乙酸乙酯粗提物对粘虫幼虫具有很强的生长发育抑制作用,处理后1,2,3,4,5,6d的生长发育抑制率分别为100%和100%,76.92%和61.54%,66.67%和59.26%,70.27%和45.95%,72.55%和49.02%,67.12%和35.62%;具有很强的麻醉作用,1h内的麻醉率分别为68.0%和66.0%,麻醉作用的回归方程分别为Y=2.0542+3.1331 X和Y=3.0114+2.3334 X,苏醒中时RT50分别为8.71和7.12h;对小菜蛾、菜粉蝶、大菜粉蝶和云斑粉蝶幼虫具有很强的触杀作用,稀释5倍液(小菜蛾4倍液)的校正死亡率分别为75.0%和58.33%,87.50%和95.0%,100%和93.33%,100%和100%;对萝卜蚜均具有很强的触杀作用,稀释30倍液的校正死亡率分别高达98.52%和97.79%。
Insecticidal activities on buds and flowers of Solanurn tuberosum were not reported at home and abroad. Study on insecticidal activities and action ways of extracts from dry buds on dark condition and flowers of S. tuberosum against Mythimna separate can provide clue and theoretical basis for formulating new type of botanical insecticide. The thesis conducted insecticidal activity of ethyl acetate extracts from dry buds on dark condition and flowers of S. tuberosum （Zhuangshu 3） by immersion and sandwich methods. The results showed that ethyl acetate extracts from dry buds and flowers had very strong contact toxicity against 5th instar larva of M. separate, the corrected mortality were 52.0% and 61.36%, respectively when the extracts were diluted 5 times. The extracts had very strong developmental inhibition action against the 3rd instar larva, developmental inhibition rates of 1, 2, 3, 4, 5 and 6 d after treatment were 100% and 100%, 76.92% and 61.54%, 66.67% and 59.26%, 70.27% and 45.95%, 72.55% and 49.02%, 67.12% and 35.62%, respectively. The extracts had very strong anaesthesia action against the larva, anaesthesia rate were 68.0% and 66.0% in one hour, regression equation were Y=2. 0542＋3. 1331X and Y=3. 0114＋2. 3334X, RT50 were 8.71 and 7.12 h. The extracts had very strong contact toxicity against larvae of Plutella xylostella, Pieris rapae, Artogeia brassicae and Pontia daplidice, corrected mortality were 75.0% and 58.33%, 87.50% and 95.0%, 100% and 93.33%, 100% and 100% （diluted 5 times, 4 times to P. xylostella ）, respectively. The extracts also had very strong contact toxicity against Lipaphis erysirni with corrected mortality of 98.52% and 97.79% （diluted 30 times）.
出处
《草业学报》
CSCD
北大核心
2013年第6期173-181,共9页
Acta Prataculturae Sinica
基金
甘肃省科学事业费项目(QS041-C31-06)
甘肃省农业生物技术研究与应用开发项目(GNSW-2005-04)资助
关键词
马铃薯(庄薯3号)
无光干芽
花
乙酸乙酯粗提物
杀虫活性
Solanurn tuberosurn （Zhuangshu 3）
dry buds on dark conmdition
flowers
ethyl acetate extracts
insecticidal activity
