摘要
用荧光光谱、紫外吸收光谱和电化学方法研究了在近似生理条件下秋水仙碱与人血清白蛋白的相互作用.研究表明,秋水仙碱对人血清白蛋白的荧光猝灭主要是静态猝灭过程,秋水仙碱使人血清白蛋白的构象发生变化.与此同时,在pH=7.4、含0.05%吐温-20的磷酸盐缓冲液中,秋水仙碱在玻碳电极上出现一不可逆的氧化峰,加入人血清白蛋白后秋水仙碱的氧化峰电位正移,峰电流下降.此外,利用光谱学方法和电化学方法测定的秋水仙碱与人血清白蛋白相互作用的结合常数和结合位点数吻合.
The interaction between colchicine and human serum albumin （HSA） under nearphysiological condition was investigated by fluorescence spectrometry, ultraviolet visible light absorption spectrometry and electrochemical method. It was found that colchicine quenches the fluorescence of HSA mainly through a static quenching process, and colchicine is able to alter the conformation of HSA. In the meantime, in the phosphate buffer solution （pH = 7.4） containing 0. 05% Tween-20, colchicine shows an irreversible oxidation peak on glassy carbon electrode, and the introduction of HSA leads to positive shift of the oxidation peak as well as decrease of the oxidation peak current of colchicine. Moreover, the binding constants and the numbers of binding sites determined by spectroscopic and electrochemical methods for colchicine and HSA are well consistent with each other.
出处
《化学研究》
CAS
2013年第6期599-604,共6页
Chemical Research
关键词
秋水仙碱
人血清白蛋白
相互作用
光谱学
电化学
colchicine
human serum albumin
interaction
spectrometry
electrochemistry
