摘要
为研究单核细胞趋化蛋白 - 1对单核细胞表面淋巴细胞功能相关抗原 - 1、清道夫受体和载脂蛋白E表达的影响 ,采用培养人单核细胞株THP - 1细胞 ,以间接免疫荧光法结合流式细胞术和激光共聚焦扫描显微镜分别检测淋巴细胞功能相关抗原 - 1和清道夫受体蛋白的表达 ,用逆转录 -多聚酶链反应检测清道夫受体和载脂蛋白EmRNA的表达水平。结果显示 ,在单核细胞趋化蛋白 - 1刺激组淋巴细胞功能相关抗原 - 1和清道夫受体蛋白的表达明显高于对照组 (P <0 .0 1) ;17- β -雌二醇可部分抑制单核细胞趋化蛋白 - 1对THP - 1细胞表面淋巴细胞功能相关抗原 - 1表达的促进作用 (P <0 .0 5 ) ,但对单核细胞趋化蛋白 - 1促进清道夫受体表达的作用无明显影响 ;单核细胞趋化蛋白 - 1明显促进THP - 1细胞清道夫受体mRNA的表达 ,而对载脂蛋白EmRNA的表达无明显影响。研究提示 ,单核细胞趋化蛋白 - 1可通过促进单核细胞表面粘附分子、淋巴细胞功能相关抗原 - 1以及清道夫受体的表达而促进动脉粥样硬化的发生 ,雌二醇还可能通过抑制单核细胞趋化蛋白 - 1对淋巴细胞功能相关抗原- 1表达的促进而发挥抗动脉粥样硬化作用。
Aim To study the effect of monocyte chemoattractant protein-1(MCP-1) and 17-β-estradiol(E2) on the expression of lymphocyte function associated antigen-1(LFA-1), scavenger receptor(SR) and apolipoprotein E(apoE) by monocytes. Methods THP-1 cells (a human acute monocytic leukemia cell line) were cultured for the experiments. The protein expression of LFA-1 and SR on THP-1 cells were assayed by indirect immunofluorescence combined with flow cytometry and confocal laser scanning microscopy respectively. The monoclonal antibody of SR was 2F8(rat anti mouse macrophage SR). The mRNA expression of SR and apoE in THP-1 cells was determined by reverse transcription-PCR(RT-PCR). Results MCP-1 could stimulate the expression of LFA-1 on THP-1 cells, the positive cell rate in MCP-1 group and control group were 66.1%±2.5% and 43.3%±3.7% (P<0.01), the mean fluoresence intensity(MFI) were 221.3±17.8 and 132.8±18.7 respectively (P<0.01). The positive cell rate and MFI in MCP-1+ E2 group were 59.5%±2.1% and 204.2±22.5 (P<0.05 versus MCP-1 group), respectively. MCP-1 also stimulated the protein expression of SR on THP-1 cells, the net fluoresence intensity(NFI) in MCP-1 group and control group were 48.9±9.8 and 32.5±3.2 respectively (P<0.01), the NFI in MCP-1+ E2 group were 49.7±10.4 (P>0.05 versus MCP-1 group). The RT-PCR also showed that MCP-1 could induce the mRNA expression of SR on monocytes, but doesn’t have obvious effects on apoE expression. [WT5”HZ]Conclusions By increasing the expression of LFA-1 on monocytes, MCP-1 can promote the monocyte-endothelium adhesion. MCP-1 also induces the expression of SR on monocytes, but doesn′t have obvious effects on apoE expression, therefore
出处
《中国动脉硬化杂志》
CAS
CSCD
2000年第4期322-326,共5页
Chinese Journal of Arteriosclerosis
