摘要
枯草芽孢杆菌D-29是一株具有广谱抑菌活性的生防菌株。对D~29菌株发酵条件进行了优化,结果表明,最佳初始培养基配方为:玉米粉10g,麦芽糖30g,碳酸钙1.5g,豆粉30g,水1000mL;最佳培养条件为:250mL三角瓶装液量100mL,初始pH值为7,接菌量1%,培养温度28℃,培养时间48h,转速170--190r/min。
Abstract:Bacillus subtilis D-29 is an antagonistic bacterium with broad-spectrum antimicrobial activity. The optimal for culture media and fermentation conditions of D-29 were determined in the study. Results showed that optimum fermentation nL component for strain Bacillus subtilis D-29 was corn flour 10 g, maltose 30 g, soybean dregs 30 g, CaC03 1.5 g, and water I 000 mt obtained best fermentation condition was described as: amount of load 100 mL (in a 250 mL flask), amount of inoculation 1%, bt pH 7, the fermentation temperature 28 ℃, incubation time 48 h, and rotation speed 170-190 r/min.
出处
《山西农业科学》
2013年第12期1333-1335,共3页
Journal of Shanxi Agricultural Sciences
基金
国家自然科学基金青年基金项目(31000873)
山西省科技攻关项目(20120311016-2)
山西农业大学中青年学术骨干基金项目(XG201210)
关键词
枯草芽孢杆菌
发酵条件
生物防治
Bacillus subtilis
fermetation conditions
biological control