摘要
背景:至今各种干细胞作为软骨组织工程种子细胞已被广泛研究。目的:分析不同干细胞的生物学特性及其在软骨组织工程中的研究与应用。方法:通过检索近年来关于软骨组织工程研究中的干细胞来源及其影响因素的相关文献,针对软骨组织工程不同来源干细胞的优化获取、存在的问题、影响细胞分化生长、繁殖及代谢的因素进行了着重论述,为组织工程化软骨修复软骨缺损提供理论基础。结果与结论:通过大量的实验研究证实外源性的干细胞可用于软骨组织工程构建的种子细胞。软骨组织工程种子细胞构建的影响因素包括生长因子、细胞支架、细胞接种密度、氧浓度、应力和微重力等。然而组织工程化软骨仍处于实验室研究阶段,软骨组织工程涉及干细胞的许多问题,如干细胞的改造、干细胞体外培养、如何延长细胞寿命、降低细胞抗原性及增强宿主免疫耐受等尚待解决。
BACKGROUND： A variety of stem cells as seed cells for cartilage tissue engineering have been extensively studied. OBJECTIVE： To analyze the biological characteristics of different kinds of stem cells and their application in cartilage tissue engineering. METHODS： By retrieving recent studies on sources of stem cells and influential factors in cartilage tissue engineering, the authors focused on optimization of harvesting stem cells from different sources in bone tissue engineering, problems, and factors that affect cell growth, differentiation, reproduction and metabolism, which provide a theoretical basis for tissue-engineered cartilage repair. RESULTS AND CONCLUSION： A large number of experimental studies have confirmed that exogenous stem cells can be used as seed cells for cartilage tissue engineering. Influential factors relative to cartilage tissue engineering construction using seed cells include growth factors, cell scaffold, cell seeding density, oxygen concentration, stress and micro-gravity. However, tissue-engineered cartilage is still in the stage of laboratory research, and stem cells for cartilage tissue engineering involves many issues, such as stem cell transformation in vitro culture of stem cells, how to prolong the survival of cells, reduce cell antigenicity and enhance the host＇s immune tolerance, which are yet to be resolved.
出处
《中国组织工程研究》
CAS
CSCD
2013年第46期8116-8121,共6页
Chinese Journal of Tissue Engineering Research
