摘要
背景:多项体内外实验表明外源性植入碱性成纤维细胞生长因子能明显促进骨形成过程,但外源性碱性成纤维细胞生长因子在体内易降解,影响疗效。目的:利用分子生物学技术将碱性成纤维细胞生长因子转染至骨髓间充质干细胞中,观察同种异体骨复合基因转染骨髓间充质干细胞修复绵羊极限骨缺损的效果。方法:将同种异体骨复合碱性成纤维细胞生长因子转染骨髓间充质干细胞组织工程骨、骨髓间充质干细胞复合同种异体支架骨材料、同种异体支架骨材料、β-磷酸三钙材料分别植入羊髂骨极限缺损处,植入后4,8,12周行组织学、免疫组织化学染色观察。结果与结论:同种异体骨复合碱性成纤维细胞生长因子转染骨髓间充质干细胞组织工程骨植入后12周,手术结合区成软骨样结构较多,术区中央可见大量成骨样细胞,整个术区的支架材料降解较其他组多,支架材料孔洞内爬满纤维结缔组织,材料周围常见破骨样细胞;骨涎蛋白与Ⅰ型胶原呈强阳性表达。其他3组手术结合区虽有成软骨样结构及成骨样细胞出现,但中央区为死骨结构,且骨涎蛋白与Ⅰ型胶原呈弱表达。表明碱性成纤维细胞生长因子转染的骨髓间充质干细胞复合同种异体骨可基本修复绵羊极限骨缺损。
BACKGROUND： Many in vivo and in vitro experiments indicate that implantation of exogenous basic fibroblast growth factor can significantly promote the process of bone formation, but the in vivo degradation of exogenous basic fibroblast growth factor affects the therapeutic efficacy.OBJECTIVE： To observe the effects of basic fibroblast growth factor-transfected mesenchymal stem cells which transfected using molecular biology techniques combined with allogeneic bone in the repair of critical-size bone defects in sheep.METHODS： Allogeneic bone with basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells, bone marrow mesenchymal stem cells combined with allogeneic bone material stents, allograft bone material, β-tricalcium calcium material were respectively implanted into critical-size bone defects in sheep. After 4,8 and 12 weeks, histological and immunohistochemical staining was performed.RESULTS AND CONCLUSION： At 12 weeks after implantation of allogeneic bone with basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells as tissue engineering bone, there were many cartilage-like structures in the operative binding region and a large amount of osteoblast-like cells in the center of operative region, and there was more material degradation in the entire operative area as compared with other groups; there were fibrous connective tissues full of the pores, and osteoclast-like cells were commonly seenaround the implant material; bone sialoprotein and collagen type Ⅰ expression were strongly positive. In the other three groups, although the cartilage-like structure appeared in the binding region, dead bone structure was found in the central area, and bone sialoprotein and type Ⅰ collagen expression was weak. These findings indicate that allogeneic bone with basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells can basically repair critical-size bone defects in sheep.
出处
《中国组织工程研究》
CAS
CSCD
2013年第47期8141-8148,共8页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金项目(81060088)
课题项目名称:三维打印构建组织工程化牙槽骨的实验研究~~
