摘要
目的建立用于测定人血清中丙戊酸钠浓度的超高效液相色谱法。方法采用超高效液相色谱法,色谱柱为ACQUITY UPLC@BEH C_(18)(50 mm×2.1 mm,1.7μm),柱温40℃,检测波长248 nm,流动相:甲醇-水(60∶40)(0min)→甲醇-水(80∶20)(4 min)→甲醇-水(100∶0)(5 min)→甲醇-水(60∶40)(8 min),流速0.5 mL·min^(-1)。结果血清质量浓度在16.632~166.320 mg·L^(-1)内线性良好。回归方程:y=0.019x+0.068 4,r=0.999 9。在低、中、高质量浓度下的回收率分别为98.473%、98.508%、94.606%,日内精密度RSD为0.290%~1.614%(n=5),日间精密度RSD为0.628%~2.522%(n=5)。结论本法灵敏、准确、快速、专属性强,适用于丙戊酸钠的血药浓度监测。
AIM To establish an ultra-high performance liquid chromatography （UPLC） method to determine sodium valproate concentration in human serum. METHODS By ultra-high performance liquid chromatography, the AC- QUITY UPLC @BEH C18（50 mm ×2.1mm,1.7μm） column was used, column temperature was 40℃, detection wave- length was 248 nm, mobile phase was methanol - water （60：40） （0 min） →methanol - water （80：20） （4 min）→ methanol- water （100：0） （5 min）→ methanol - water （60：40） （8 min） and flow rate was 0.5 mL·L^-1. RESULTS There was good linearity within the range of 16. 632 - 166. 320 mg·L^-1. The regression equation was y = 0.019x ＋ 0. 068 4, r = 0. 999 9. In the low, medium and high concentrations recoveries were 98.473 %, 98. 508%, 94.606%, re- spectively. The intra-day precision was 0. 290% - 1. 614% （ n = 5）, and the inter-day precision was 0. 628% - 2.522% （ n = 5）. CONCLUSION The method is sensitive, accurate, rapid and specific, and suitable for sodium val- proate blood concentration monitoring.
出处
《中国临床药学杂志》
CAS
2013年第6期342-345,共4页
Chinese Journal of Clinical Pharmacy
基金
安医大二附院博士科研启动基金(编号NO 2012BKJ022)
关键词
超高效液相色谱法
丙戊酸钠
血药浓度监测
ultra-high performance liquid chromatography
sodium valproate
blood concentration monitoring
