摘要
目的:研究新型脂肪细胞因子Chemerin对人外周血单核细胞黏附功能,以及跨内皮细胞迁移功能的影响。方法:人脐静脉内皮细胞体外分离培养;采用流式细胞分选技术分离人外周血CD14阳性单核细胞;单核细胞与脐静脉内皮细胞共培养的方法研究不同浓度重组人Chemerin对单核细胞黏附功能的影响;Transwell方法研究不同浓度重组人Chemerin对单核细胞跨内皮细胞迁移功能的影响。结果:重组人Chemerin能显著促进单核细胞与血管内皮细胞的黏附以及跨内皮细胞迁移,并且作用随着Chemerin浓度的增加而增强(黏附的单核细胞数对照组(4.00±3.37)视野,Chemerin 100μg/L(26.75±4.57)视野,200μg/L(32.25±16.38)视野,300μg/L(48.25±19.50)视野,与对照组比较P值分别为0.006、0.001及0.0001;迁移的细胞数对照组为(0.763±0.042)×104个,100μg/L组(1.17±0.153)×104个,200μg/L组(1.60±0.100)×104个,500μg/L组(1.87±0.058)×104个,与对照组比较,均P<0.001;Chemerin中和抗体能显著抑制Chemerin促单核细胞黏附及迁移的作用(P<0.05)。结论:单核细胞黏附以及继发迁移在冠心病发生发展过程中起重要作用,重组人Chemerin能显著促进人外周血单核细胞与血管内皮细胞的黏附,以及跨血管内皮细胞迁移,提示Chemerin通过影响单核细胞功能参与了动脉粥样硬化的发生发展。
Objective：Monocyte adhesion and transendothelial migration play a key role in atherosclero- sis. Here we study the effect of Chemerin on adhesion and transendothelial migration of human monocytes. Methods： Human umbilical vein endothelial cells （HUVECs） were cultured in vitro. Human CD14＋ mouocytes were isolated by flow cytometry technique. To study the effect of ehemerin on adhesion of monoeytes in the meth- od of monocytes and HUVECs co-culture, and to study the effect of chemerin transendothelial migration of mon- ocytes in the method of transwell migration. Results：The recombinant human Chemerin can significantly pro- mote monocytes adhesion and transendothelial migration in a dose-dependent manner which could be inhibited by chemerin neutralizing antibody （ P 〈 0. 05 ） （ the number of adherent monocytes ： control group （ 4. 00 ± 3. 37 ） vision, Chemerin 100ng/mL （ 26. 75 ± 4. 57 ） vision, 200 μg/mL （ 32. 25 ± 16. 38 ） vision, 300 μg/mL （48.25 ± 19. 50） vision, P were 0. 006, 0. 001, 0. 000 respectively, compared with the control group;the a-mount of migrated monoyctes ：the blank control （0. 763± 0. 042 ）× 10^4 cells, 100ng/mL （1.17±0. 153 ） × 10^4 （sells, 100ng/L （[.（30 ±0. 100） ×l0^4 cells, 500 μg/L （l. 87 ±0.058） × 10^4 cells, P values all were less than 0. 001 compared with the blank control）. Conclusion：Monocyte adhesion and transendothelial migration play a key role in atheroselerosis. Recombinant human Chemerin can significantly promote human monoeytes ad- hesion and transendothelial migration. It suggests that Chemerin may be involved the occurrence and develop- ment of atherosclerosis by influencing the function of monocytes.
出处
《心肺血管病杂志》
CAS
2013年第6期777-782,共6页
Journal of Cardiovascular and Pulmonary Diseases
基金
国家自然科学基金项目(81100600)
