摘要
目的 采用氟与微量元素联合配制成以钙、磷为基础 ,含有氟和微量元素的矿化液 ,探讨该矿化液对致龋毒力因子——变形链球菌和远缘链球菌的胞外葡糖基转移酶 (GTF)和乳酸脱氢酶 (L DH )的影响。方法 选用国际标准菌株 ATCC S.mutans 2 5 175和 S.Sobrinus6 715为实验菌侏 ,采用厌氧菌的连续培养技术培养细菌 ,用微量酶化学分析方法检测矿化液对 GTF和 L DH酶活性的影响 ,用菌液的吸光度(ABS)来描述。结果 无氟矿化液对 GTF酶活性无明显的抑制作用 (P>0 .0 5 ) ,而含氟矿化液和氟微量元素矿化液对 GTF酶活性有明显抑制作用 (P<0 .0 1) ,两两比较 ,尤以氟微量元素矿化液的作用最强 ;氟微量元素矿化液对 L DH酶活性具有明显的抑制作用 (P<0 .0 1) ,而无氟矿化液和含氟矿化液对其无明显的统计学意义 (P>0 .0 5 )。结论 氟微量元素矿化液比单氟矿化液具有更好的抑制致龋毒力因子 GTF和L DH酶活性的作用。提示氟与微量元素的联合应用 ,是其有效的防龋途径之一 ,同时在调节菌斑生态平衡中起重要作用。
Objective:Using a kind of special mineral solution,which is on the basis of calcium and phosphate,added in fluoride and trance element,this study is aimed to investigate its influence on the cariongenic factor-the extracellular GTF and LDH produced by S.Mutuns and S.Sobrinus of Mutans Streptococci.Methods:Internationally standarded strains AYCC S.mutans 25175 and S.Sobrinus 6715 were selected as test strains(TS).Usingthe method of continual anaerobic incubation to culture is and micro-chemistry enzyme analysis technique to detect the influence of the mineral solution on the enzyme activity of GTF and LDH,the enzyme activity is described by ABS of the bacterial solution.Results:Non-fluoridated mineral solution has no obvious inhibitive effect on the enzyme activity of GTF( P >0.05),while the fluoridated solution and the fluoridated solution containing trace element do ( P >0.01),and thd latter has the most marked effect.Moreover,it also has obvious inhibitive effect on the enzyme activity of LDH( P <0.01),while the other two remineralization solution don′t( P >0.05).Conclusion:The fluoridated remineralization solution containing trace element has stronger inhibitive effect on the enzyme activity of GTF and LDH than simply fluoridate mineral solution.This study suggest:application of the effective ways to prevent caries,and is one of the dffective ways to prevent caries,and it also plays an important role in regulating the ecological balance of dental plague.
出处
《中国微生态学杂志》
CAS
CSCD
2000年第5期292-294,共3页
Chinese Journal of Microecology
