摘要
目的研究结直肠癌患者血清瘦紊和癌组织中受体与临床特征的关系。方法采取50例结直肠癌患者和42例健康对照者新鲜血清,应用酶联免疫吸附试验测定瘦素的浓度,并且收集结直肠癌患者癌组织及癌旁正常组织样本,运用免疫组化的方法检测瘦素受体的表达,并分析其间是否存在差异。结果共有直肠(16例)、左半结肠(12例)、右半结肠(22例),按肿瘤TNM分期,Ⅰ/Ⅱ期组(22例),Ⅲ/Ⅳ期组(28例)。肿瘤组患者的血清瘦素水平明显高于对照组;不同位置肿瘤患者血清瘦素表达有明显差异;晚期肿瘤组瘦素水平高于早期组;有远处转移组的血清瘦素水平明显高于未转移组;超重组血清瘦素水平高于正常体重组;瘦素水平与患者性别,肿瘤分化没有明显相关。瘦素受体在结直肠癌组织和正常结直肠组织中均有表达,癌组织的表达阳性率和表达强度均高于正常结直肠组织;晚期癌组织瘦素受体表达阳性率和表达强度高于早期癌组织;但瘦素受体与结直肠癌组织分化,位置,体重指数无明显相关。结论瘦素可能有促进结直肠癌的侵袭和远处转移的作用,另外瘦素在肥胖与结直肠癌的关系中可能起了重要作用。
Objective To investigate serum leptin levels and tissue expression of leptin receptor and their association with clinicopathological characteristics of colorectal cancer patients. Methods Serum samples were col ected from 50 patients with colorectal cancer and 42 healthy subjects;tissue specimens of colorectal cancer and corresponding pericancerous mucosa were also col ected from patients. The serum leptin levels were measured by ELISA and the expression of leptin receptors were detect-ed by immunohistochemistry. Results Serum leptin concentrations of colorectal cancer patients were significantly higher than those of healthy controls.Serum leptin levels were higher in patients with advanced stages than those in early stages, in patients with distant metastases than those without metastases, in patients with overweight than those with normal weight. However, there was no difference in serum leptin levels between male and female and between patients with different tumor differentiations..The expression of leptin receptor was higher in tumor tissue than that in pericancerous tissue, in patients with more advanced stage than in early stage;while the expression of leptin receptor was not associated with location and differentiation of tumor, and BMI of patients. Conclusion Leptin may be associated with the progress and metastasis of colorectal cancer, which may also be corre-lated with obesity of patients.
出处
《浙江医学》
CAS
2013年第21期1875-1878,共4页
Zhejiang Medical Journal
基金
杭州市医药卫生科技计划项目(2008A008)
关键词
瘦素
瘦素受体
结直肠癌
肥胖
Leptin Leptin receptor Colorectal cancer Obesity
