摘要
目的分析不同宫颈病变患者高危HPV DNA及其E6/E7mRNA在不同宫颈病变中的阳性率有无差异,评估mRNA拷贝数大小与病变等级的关系。方法采用聚合酶链反应-反向点杂交技术、原位杂交技术对101例宫颈脱落细胞标本(炎症组29例,低级别组29例,高级别组43例)进行HPV分型及高危HPV E6/E7 mRNA拷贝数的检测。结果高危HPV DNA及其E6/E7mRNA在炎症组与低级别组、炎症组与高级别组中的阳性率均具有统计学差异(均P<0.05),其拷贝数的均值随着宫颈病变等级的上升而明显增加。结论高危HPV的感染及其E6/E7mRNA的表达与宫颈病变密切相关,E6/E7 mRNA拷贝数的大小影响宫颈病变的程度。两者结合将更有利于对宫颈病变的综合评估。
Objective To detect high- risk HPV DNA and expression of HPV E6/E7mRNA in patients with different cervi-cal lesions. Methods Samples were collected from patients with cervical inflammation （n=29）, low grade cervical intraepithelial neoplasia （CIN, n=29） and high grade CIN（n=43）, HPV DNA and HPV E6/E7 mRNA were detected by PCR- reverse point hybrid technology and in situ hybridization technique. Results The positive rate of high- risk HPV DNA and E6/E7 mRNA in inflammato-ry group and low grade CIN,inflammatory group and high grade CIN were both statistical y significant.The E6/E7 mRNA tran-scripts were increasing when cervical lesion developped. Conclusion High risk HPV infection and HPV E6/E7mRNA expression are closely correlated with cervical lesions and the detection of both parameters is beneficial for comprehensive assessment of cervical lesions.
出处
《浙江医学》
CAS
2013年第21期1897-1899,共3页
Zhejiang Medical Journal
