摘要
目的:探讨受激活调节正常T细胞表达和分泌因子(RANTES)受体CCR1、CCR5在成人附睾中的表达和定位。方法:采用RT-PCR检测CCR1和CCR5 mRNA在成人附睾中的表达,免疫组织化学法观察CCR1和CCR5在人附睾中的细胞定位,免疫荧光双标染色分别检测RANTES与CCR1及CCR5的共定位情况。结果:在人附睾组织中获得了RANTES受体CCR1、CCR5的cDNA片段,免疫组织化学显示CCR1表达于输出小管的纤毛细胞,附睾管的顶细胞和基细胞;CCR5表达于附睾输出小管的纤毛细胞以及全部附睾管上皮细胞。免疫荧光双标显示RANTES分别与CCR1和CCR5的阳性信号在输出小管的纤毛细胞、附睾管的顶细胞和基细胞共存。结论:CCR1和CCR5在附睾上皮有表达,且与RANTES共定位,推测RANTES可能通过其受体在附睾中起作用,从而为精子成熟和储存提供适宜的微环境。
Objective： To explore the expression and the cell localization of regulated upon activation normal T cell expressed and secreted （RANTES） receptors CCR1 and CCR5 in human epididymis. Methods： RT-PCR was used to detect the mRNA expression of CCR1 and CCR5 in human epididymis. Immunohistochemical staining was employed to display the distribution of CCR1 and CCR5 in human epididymis. The colocalization of RANTES and CCR1 or CCR5 in epididymis was observed by immunofluorescence double staining method. Results： The RT- PCR products of the predicted sizes for CCR1 and CCR5 were acquired from human epididymis. Immunohis- tochemical study demonstrated that CCRl-positive signal was identified in ciliated cells of the efferent ducts, apical and basal cells of the epididymal ducts, whereas CCR5 was located in ciliated cells of the efferent ducts and all epithelial cells of epididymal ducts. Double-labelling analysis indicated that immunofluorescence of RANTES coincided with CCR1 or CCR5 in ciliated cells, apical and basal cells of epididymis. Conclusion： CCR1 and CCR5 are expressed in the human epididymal epithelium and coincide with RANTES. According to the experimental results, it is assumed that RANTES may play a role in epididymis via receptors CCR1 and CCR5, thereby providing a suitable environment where sperm cell can be matured and stored.
出处
《生殖与避孕》
CAS
CSCD
2013年第12期791-798,共8页
Reproduction and Contraception
基金
国家自然科学基金资助项目
项目号:81170614
