期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

核酸技术在乳腺癌实验诊断中的进展 被引量：3

Advance of the nucleic acid techniques in the breast cancer diagnostics
原文传递
导出
摘要 乳腺癌的实验室诊断传统策略主要是基于抗原-抗体反应的免疫学诊断,如CA-153、细胞角蛋白CK8/18/19等;以及基于核酸（DNA/mRNA）的分子诊断,如BRCA1/2、HER2等.本文阐述了循环DNA、循环miRNA、核酸适配子、循环肿瘤细胞等基于核酸策略的新兴技术在乳腺癌实验诊断中的进展. Traditional immunological strategy which largely based on antigen-antibody techniques such as CA153,cytokeratin CK8/18/19,is still the main diagnostic biomarkers for breast cancer.Molecular biology markers such as BRCA1/2 and HER2 are becoming popular and hot.This article summarizes recent progress of breast cancer diagnosis based on nucleic acid related strategies such as circulating DNA,miRNAs,aptamers and circulating tumor cells.
作者 汪付兵 涂建成
机构地区 武汉大学中南医院检验科
出处 《中华检验医学杂志》 CAS CSCD 北大核心 2013年第11期978-982,共5页 Chinese Journal of Laboratory Medicine
基金 国家重点基础研究发展规划“973”项目（2012CB720600）子课题（2012CB720605）
关键词 乳腺癌 循环DNA MIRNA 核酸适配子 循环肿瘤细胞 Breast cancer Circulating DNA/miRNAs Aptamers Circulating tumor cells
分类号 R737.9 [医药卫生—肿瘤]
  • 相关文献

参考文献47

  • 1Wiechmann L,Sampson M,Stempel M,et al.Presenting features of breast cancer differ by molecular subtype.Ann Surg Oncol,2009,16:2705-2710.
  • 2Roth C,Pantel K,Muller V,et al.Apoptosis-related deregulation of proteolytic activities andhigh serum levels of circulating nucleosomes and DNA in bloodcorrelate with breast cancer progression.BMC Cancer,2011,11:4.
  • 3Page K,Hava N,Ward B,et al.Detection of HER2 amplifi cation in circulating free DNA in patients with breast cancer.Br J Cancer,2011,104:1342-1348.
  • 4刘景辉,王昊,徐立红.胞外DNA的研究进展[J].中国细胞生物学学报,2013,35(1):59-70. 被引量：3
  • 5Polcher M,Ellinger J,Willems S,et al.Impact of the menstrual cycle on circulating cell-free DNA.Anti Cancer Res,2010,30:2235-2240.
  • 6Shaw JA,Brown J,Coombes RC,et al.Circulating tumor cells and plasma DNA analysis in patients with indeterminate early or metastatic breast cancer.Biomark Med,2011,5:87-91.
  • 7Dawson SJ,Tsui DW,Murtaza M,et al.Analysis of circulating tumor DNA to monitor metastatic breast cancer.N Engl J Med,2013,368:1199-1209.
  • 8Gevensleben H,Garcia-Murillas I,Graeser MK,et al.Noninvasive detection of HER2 amplification with plasma DNA digital PCR.Clin Cancer Res,2013,19:3276-3284.
  • 9Liggett TE,Melnikov AA,Marks JR,et al.Methylation patterns in cell-free plasma DNA reflect removal of the primary tumor and drug treatment of breast cancer patients.Int J Cancer,2011,128:492-499.
  • 10Radpour R,Barekati Z,Kohler C,et al.Hypermethylation of tumor suppressor genes involved in critical regulatory pathways for developing a blood-based test in breastcancer.PLoS One,2011,6:e16080.

二级参考文献35

  • 1杨华伟,曹骥,杨南武,刘剑仑,张传珉,陈建思,洪坚善,蒋奕,苏建家.乳腺癌患者外周血SBEM mRNA的检测及其临床意义[J].癌症,2005,24(7):842-845. 被引量：8
  • 2鞠少卿,王旭东,王跃国,仲人前,王惠民.悬浮阵列技术在研究与临床中的应用[J].中华检验医学杂志,2006,29(5):393-396. 被引量：10
  • 3孙凯,汪谦.液相芯片技术在小分子RNA检测分析中的应用[J].中华医学杂志,2006,86(20):1437-1439. 被引量：14
  • 4严子禾,潘世扬,陈丹,魏源华,高丽,谢而付,黄珮珺,戎国栋,胡宜蘅,童明庆.4种血浆游离DNA提取方法的比较[J].临床检验杂志,2006,24(5):363-365. 被引量：27
  • 5Husemlann Y, Geigl JB, Schubert F, et al. Systemic spread is an early step in breast cancer. Cancer Cell, 2008,13:58-68.
  • 6Maheswaran S, Haber DA. Circulating tumor cells : a window into cancer biology and metastasis. Curr Opin Genet Dev, 2010,20: 96-99.
  • 7Ring A, Smith IE, Dowsett M. Circulating tumour cells in breast cancer. Lancet Oncol, 2004,5:79-88.
  • 8Deng G, Herrler M, Burgess D, et al. Enrichment with anti- cytokeratin alone or combined with anti-EpCAM antibodies significantly increases the sensitivity for circulating tumor cell detection in metastatic breast cancer patients. Breast Cancer Res, 2008,10 : R69.
  • 9Allard WJ, Matera J, Miller MC, et al. Tumor cells circulate in the peripheral blood of all major carcinomas but not in healthy subjects or patients with nonmalignant diseases. Clin Cancer Res, 2004,10:6897-6904.
  • 10Fehm T, Sagalowsky A, Clifford E, et al. Cytogenetic evidence that circulating epithelial ceils in patients with carcinoma are malignant. Clin Cancer Res, 2002, 8:2073-2084.

共引文献23

同被引文献24

  • 1Vather R, Sammour T, Kahokehr A, et al. Lymph node evaluation and long-term survival in Stage II and Stage III colon cancer: a national study[ J]. Ann Surg Oncol, 2009, 16: 585- 593.
  • 2Zhang X, Wang C, Wang L, et al. Detection of circulating Bmi-1 mRNA in plasma and its potential diagnostic and prognostic value for uterine cervical cancer[J]. Int J Cancer, 2012, 131: 165- 172.
  • 3王传新,张欣.一种检测宫颈癌血浆Bmi-1mRNA的专用试剂盒及方法[P].中国专利:201110167829,2011-11-02.
  • 4Kim JH, Yoon SY, Kim CN, et al. The Bmi-1 oncoprotein is overexpressed in human colorectal cancer and correlates with the reduced pl6INK4a/pl4ARF proteins [ J ]. Cancer Lett, 2004, 203 : 217-224.
  • 5He X, Dang Y, Wu CW, et al. MicroRNA-218 inhibits cell cycle progression and promotes apoptosis in colon cancer by downregulating BMI1 polycomb ring finger oncogene [ J ]. Mol Med, 2013, 18: 1491-1498.
  • 6Liu YL, Jiang SX, Yang YM, et al. USP22 acts as an oncogene by the activation of BMI-l-mediated INK4a/ARF pathway and Akt pathway[J]. Cell Biochem Biophys, 2012, 62: 229-235.
  • 7Du J, Li Y, Li J, Zheng J. Polycomb group protein Bmil expression in colon cancers predicts the survival[ J]. Med Oncol, 2010, 27: 1273-1276.
  • 8Silva J, Silva JM, Garcia V, et al. RNA is more sensitive than DNA in identification of breast cancer patients bearing tumor nucleic acids in plasma[ J]. Genes Chromosomes Cancer, 2002, 35 : 375-376.
  • 9Tichopad A, Kitchen R, Riedmaier I, et al. Design and optimization of reverse-transcription quantitative PCR experiments [J]. Clin Chem, 2009, 55: 1816-1823.
  • 10Asaga S, Kuo C, Nguyen T, et al. Direct serum assay for microRNA-21 concentrations in early and advanced breast cancer [J]. ClinChem, 2011,57: 84-91.

引证文献3

二级引证文献18

中华检验医学杂志
2013年 第11期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部