摘要
氯酚类有机物属于高毒性、难降解有机污染物,在自然环境中分布广泛.本文以2,4-二氯苯酚(2,4-DCP)为氯酚类物质的典型代表,以硫酸盐还原菌(SRB)为出发菌株,通过改变培养基中2,4-DCP的浓度,观测菌体浓度、硫酸盐还原速率和菌体形貌等的变化,探讨不同浓度2,4-DCP对SRB生长和代谢活性的影响.结果发现,在整个培养过程中2,4-DCP浓度基本没有发生变化,表明纯培养SRB并没有降解转化2,4-DCP,然而2,4-DCP存在却对SRB生长和代谢活性产生两个方面的重要影响.当2,4-DCP浓度为20mg/L时,SRB生长和代谢受到抑制;2,4-DCP浓度为50mg/L时,SRB基本失活;然而,当2,4-DCP浓度为2mg/L、5mg/L时SRB生长速率加快,与空白对照相比,SRB最大生长速率提高了160.83%,代谢还原硫酸盐的速率提高了82.48%.这一结果对认识厌氧沉积环境中难生化降解有机污染物对微生物代谢活性的影响具有重要意义.
Chlorophenols （CPs）, as important contaminants widely distributed in the natural environment, are highly toxic and difficult to be biodegraded. In this paper, 2,4-dichlorophenol （2,4-DCP） was selected as a typical representative of the CPssulfate reducing bacteria （SRB） was selected as original strain. A study was conducted on the effect of 2,4-DCP on SRB’s growth and metabolic activity under different concentrations by observing the changes in cell concentration of sulfate-reducing bacteria （SRB） and sulfate reduction rates and bacterial morphology. It was found that 2,4-DCP concentration basically did not change during the whole incubation, which showed that pure cultures of SRB didn’t degrade 2,4-DCP. However, the result also showed that 2,4-DCP had important influence on the growth and metabolic activity of SRB. When the concentration of 2,4-DCP was 20mg/L, SRB’s growth and metabolism was inhibited; as the concentration come to 50mg/L, SRB was inactivated; However, while the concentration achieved 2 mg/L, 5mg/L, the growth rate of SRB was accelerated. Compared with the blank control, the maximum growth rate raised 102.67%, and sulfate-reducing rate increased 64.39%. This result has very important significance to understand the effect of organic pollutants difficultly biodegraded under anaerobic sedimentary environment on microbial metabolic activity.
出处
《南京大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第6期725-731,共7页
Journal of Nanjing University（Natural Science）
基金
973计划预研项目(2011CB411904)
国家自然科学基金(41130206
41102214
41072036
40902019)
关键词
2
4-二氯苯酚
硫酸盐还原菌
代谢活性
2,4-dichIorophenol, sulfate reducing bacteria, metabolic activity
