摘要
采用反相高效液相色谱法建立测定猕猴桃果实中的有机酸分析方法,最终确定了适用于猕猴桃中各种有机酸含量测定的色谱条件为,色谱柱:Hypersil C18(4.6 mm I.D.×250 mm,5μm);紫外检测波长:210 nm;流动相:10 mmol/L H2SO4溶液(pH2.6);流速:0.5 mL/min;柱温:30℃。结果表明,在该条件下,酒石酸,奎宁酸,苹果酸,柠檬酸和抗坏血酸等10种有机酸都得到了有效分离,方法的RSD为0.45%～1.52%,回收率为96.7%～101.2%,并测得3种猕猴桃品种果实中主要的有机酸组成为抗坏血酸、柠檬酸、苹果酸、酒石酸和奎宁酸。
A method for simultaneous determination of 10 organic acids in kiwifruit pulps by reverse phase high performance liquid chromatography was developed.Hypersil C18 column（4.6 mm I.D.×250 mm,5 μm） was used at 30 ℃ and mobile phase was 10 mmol/L H2 SO4（pH 2.6） with flow rate of 0.5 mL/min.Detection wavelength was at 210 nm.The relative standard deviations of method were 0.45 %-1.52 % and the recoveries of standards were 96.7 %-101.2 %.The results mentioned above indicated that the method was simple,rapid and accurate.It has been applied to the determination of organic acids in kiwifruit,which contained antiscorbic acid,tartaric acid,malic acid,citric acid and quininic acid.
出处
《食品研究与开发》
CAS
北大核心
2013年第19期85-87,共3页
Food Research and Development
基金
陕西省科学技术研究发展计划项目(2012k01-26)
西北农林科技大学博士科研启动基金(2010B5JJ052)
关键词
猕猴桃
反相高效液相色谱法
有机酸
kiwifruit
reverse phase high performance liquid chromatography
organic acids
