摘要
目的检测汉坦病毒感染BALB／c小鼠组织中特异性抗原及病毒RNA，建立汉坦病毒感染动物的评价体系。方法将1000LD50的汉坦病毒悬液经肌肉注射感染BALB／c小鼠，在感染后的第3天，分别取小鼠的心、肝、脾、肺、肾、脑等组织研磨后制成病毒悬液，以ELISA法和qRT—PCR法分别检测各组织中的汉坦病毒特异性抗原及病毒RNA。结果BALB／c小鼠感染汉坦病毒后短期内在脑和肝组织中可以检测到大量汉坦病毒特异性抗原以及病毒RNA，而心、脾、肺、肾组织中未检测到特异性抗原及病毒RNA。结论实验结果为建立汉坦病毒感染动物模型的评价体系提供了参考依据。
Objective To establish an evaluation system about animals infected with hantavirus, an observation of the BALB/c mice infected with hantavirus was made. Methods BALB/c mice were in- fected with hantavirus by intramuscular injection with stock solution . The specific antigen from BALB/c mice tissues after 3 days was detected with enzyme-linked immunosorbent assay （ELISA） and viral RNA with real-time polymerase chain reaction （RT-PCR）. Results Within a short term, the specific antigen and viral RNA were detected from the brain and liver at day 3 after infection, but not be detected from the heart, spleen, lung, and kidney samples. Conclusions＇ The results provided ones with some information on animals infected with hantavirus.
出处
《中国医师杂志》
CAS
2013年第11期1441-1445,共5页
Journal of Chinese Physician
基金
基金项目：国家自然科学基金（31070810
81001344）
