摘要
目的探讨自发性蛛网膜下腔出血（SAH）后血清和脑脊液细胞因子水平及其与全身炎症反应综合征（SIRS）和多器官功能障碍综合征（MODS）关系。方法选用双抗体夹心酶联免疫吸附试验（ELISA）检测自发性SAH患者血清和脑脊液IL-1β、IL--6和TNF-α浓度。结果自发性蛛网膜下腔出血后血清和脑脊液IL-1β、IL-6和TNF-α水平均显著升高（P〈0．01），但增高时段不同。血清IL-1β、IL-6和TNF-α水平与SIRS和MODS的发生有关（P〈0．05）；脑脊液IL-1β、IL-6水平与SIRS和MODS的发生有关（P〈0．05），但TNF—α水平与SIRS及MODS无关（P〉0．05）。结论自发性SAH患者血清IL-1β、IL-6和TNF-α水平及脑脊液IL-1β、IL-6水平增高可能与SIRS及MODS有关，检测自发性SAH患者血清IL-1β、IL-6和TNF—α水平及脑脊液IL-1β、IL-6水平可为预测SIRS和MODS及指导治疗提供依据。
Objective To explore the levels of IL-1β, IL-6 and tumor necrosis factor -α（TNF -α） in serum and cerebrospinal fluid of patients with spontaneous subarachnoid hemorrhage and their relationship with systemic inflammatory response syndrome （SIRS） and multiple organ dysfunction syndrome （MODS）. Methods The levels of interleukin-1β（ IL-1β） , interleukin-6（ IL-6）, and TNF α in serum and cerebro- spinal fluid of patients with spontaneous subarachnoid hemorrhage were measured with enzyme-linked immu- nosorbent assay （ELISA）. Results The levels of IL-1β, IL-6, and TNFoL in serum and cerebrospinal fluid of patients with spontaneous subarachnoid hemorrhage were significantly higher than those of control group （ P 〈 0. 05 ）, but the increased time of these cytokines was different. Three cytokines in serum and the cere- brospinal fluid levels of IL-1β and IL-6 but not TNFα were significantly related to SIRS and MODS. Con- clnsions The increased cytokine levels in serum and cerebrospinal fluid of patients with spontaneous sub- arachnoid hemorrhage may be related to SIRS and MODS, and the measurement of IL-1 β, IL-6, and TNF-α in serum, and IL-1β and IL-6 in cerebrospinal fluid of patients with spontaneous subarachnoid hemorrhage can be useful to predict and treat SIRS and MODS.
出处
《中国医师杂志》
CAS
2013年第11期1466-1469,共4页
Journal of Chinese Physician
基金
基金项目：韶关市科技计划项目（韶科卫2011.19）
韶关市医药卫生科研计划项目（Y11018）
